HOME >> BIOLOGY >> NEWS
News tips from the Journal of Neuroscience

1. Docking and Priming with Munc18
Attila Gulys-Kovcs, Heidi de Wit, Ira Milosevic, Olexiy Kochubey, Ruud Toonen, Jrgen Klingauf, Matthijs Verhage, and Jakob B. Srensen

This week, Gulys-Kovcs et al. unmask a dual role for Munc18 in docking and priming of vesicles in chromaffin cells. In vitro, Munc18 binds the "closed" state of syntaxin1 and thus occludes binding of the SNARE proteins SNAP-25 and synaptobrevin. However, Munc18 cannot be simply a negative regulator of exocytosis because vesicles fail to dock in its absence. To address this issue, the authors expressed mutant variants in chromaffin cells from Munc18 null mice and used uncaging of calcium to trigger release of primed vesicles. Expression of the NV mutation that prevents binding to closed syntaxin1 reduced vesicle docking. However, Munc18-1 NV rescued release, albeit to a lesser extent than wild type. Thus, Munc18 regulates a postdocking (priming) step by a mechanism that does not require binding to syntaxin1. In fact, the authors suggest that this second interaction either involves dissociation from syntaxin or a conformational change in the N-terminal domain of Munc18.


2. Pak1 and Neuronal Polarity
Tom Jacobs, Frdric Causeret, Yoshiaki V. Nishimura, Mami Terao, Adele Norman, Mikio Hoshino, and Margareta Nikolic

If you want to make axons, you will need some activated p21-activated kinase (Pak1), according to Jacobs et al. The authors found this CdC42 and cofilin effector in all neurites, but it was activated locally in nascent axons. In cultured hippocampal and cortical neurons, the levels of membrane-associated phosphorylated active) Pak1 peaked at the same time that neurons became polarized with distinct axons and dendrites, at ~ 2--4 d in Vitro (DIV). Total Pak1, in contrast, reached a plateau at 4 DIV and beyond. Total and activated Pak1 were evenly distributed among multiple neurites before polarization, but once a neurite
'"/>

Contact: Sara Harris
sharris@sfn.org
202-962-4000
Society for Neuroscience
7-Aug-2007


Page: 1 2 3

Related biology news :

1. News tips from the Journal of Neuroscience
2. News tips from the Journal of Neuroscience
3. News tips from the Journal of Neuroscience
4. Tips from the Journals of the American Society for Microbiology
5. Journalists can register now for ECCO 14 -- the European Cancer Conference
6. Journal of Alzheimers Disease achieves significant impact factor
7. News tips from the Journal of Neuroscience
8. Highlights from the July 2007 Journal of the American Dietetic Association
9. News tips from the Journal of Neuroscience
10. Journal of Nuclear Medicines impact grows, remains consistently high over past 5 years
11. News tips from the Journal of Neuroscience

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/7/2020)... ... January 06, 2020 , ... Myant Inc. , the pioneers ... Vegas) to bring to life its vision of a world where textile-based interfaces knitted ... the AI-powered digital sphere, and the physical IoT-enabled environment. The experience at Myant’s booth ...
(Date:12/30/2019)... SAN DIEGO (PRWEB) , ... December 30, 2019 , ... ... cooking events company has seen its highest sales in 2019, having hosted a multitude ... and Illumina, have all visited the company’s La Jolla-based venue, and the company has ...
(Date:12/18/2019)... ... December 17, 2019 , ... EconoFact, a non-profit media initiative ... Fletcher School, Tufts University, is pleased to announce a collaboration with Macmillan Learning ... using Macmillan Learning’s acclaimed series of economics textbooks. , Under the agreement, Macmillan ...
Breaking Biology News(10 mins):
(Date:1/8/2020)... ... 08, 2020 , ... Anomet Products has introduced ... use in harsh environments inside or outside of the human body. , ... alloys to achieve specific properties such as corrosion-resistance, biocompatibility, or operation at temperatures ...
(Date:1/7/2020)... ... 07, 2020 , ... Vuja De Sciences (“Vuja De”), a biotechnology startup dedicated ... appointments of Chand Khanna, DVM, PhD, DACVIM (Onc), and Dr. Lee Helman, MD, to ... are delighted to welcome Chand and Lee as new directors. They will be of ...
(Date:1/7/2020)... ... January 06, 2020 , ... ABCT ... emerging biosciences ventures invited to participate in the annual program. Twelve ventures will ... plans and a professional network. All startups will receive an entrepreneur coach, access ...
(Date:12/30/2019)... ... December 30, 2019 , ... Leak ... instruments for the Pharmaceutical, Biotechnology, Medical Device and Food Packaging Industries is pleased ... 11 compliant software program specific to the custom-built helium leak testing systems it ...
Breaking Biology Technology:
Cached News: