Johns Hopkins researchers have found a way to directly observe cell migration -- in real time and in living tissue. In a report in the June 5 issue of Developmental Cell, the scientists say their advance could lead to strategies for controlling both normal growth and the spread of cancer, processes that depend on the programmed, organized movement of cells across space

The stars of our live show are a cluster of fruit fly cells that literally crawl across the length of an egg chamber while it is maturing, says Denise Montell, Ph.D., professor of Biological Chemistry and director of the Center for Cell Dynamics. What these border cells are doing resembles what cancer cells do when they migrate from the main growth to other tissues.

The historic problem, Montell says, is that its been impossible to watch this process inside the ovarian tissue no microscope can do that and worse, the cells stop moving if theyre outside the ovaries. In a years-long effort, Montell and her team figured out just what to feed these cells to keep them alive and doing their thing without their ovarian homes.

We were stuck having to watch this through a series of still pictures and losing a lot of the story, Montell says. Now with real-time movies, were deciphering the nuances behind organized cell movement that should offer opportunities for hopefully regulating the process.

Among the nuances identified is that just like migrating geese or a pack of bicyclists, individual border cells in the cluster each take turns as the leader during their journey across the egg chamber. Another discovery is that a protein called Kuzbanian is necessary to help border cells detach from the egg wall and begin their journey. We used to think that Kuzbanian allowed border cells to squeeze themselves between other cells as they moved, Montell says, but only now do we understand the real reason cells couldnt move. We can see them valiantly trying to detach from the
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Contact: Nick Zagorski
nzagors1@jhmi.edu
443-287-2251
Johns Hopkins Medical Institutions
8-Jun-2007