Researchers have capitalized on the unique properties of a sperm cell to follow cell membrane fusion as it occurs during fertilization, tracking the full cascade of events for the first time. The findings could reveal new ways to enhance or block fertilization, as well as how to control the secretion of neurotransmitters and hormones such as insulin.

Luis Mayorga, a Howard Hughes Medical Institute (HHMI) international research scholar, and colleagues at the National University of Cuyo School of Medicine in Mendoza, Argentina, took advantage of the cellular specialization that gives sperm one irreversible chance to fertilize an egg.

The group followed the sperm's secretion of the enzymes used to penetrate the protective outer coating that surrounds an egg. "Because the sperm has a single opportunity, this secretion has to be very well-regulated," said Mayorga. "If the sperm doesn't respond right on time, it won't get through the egg's coating." And since fertilization is one-way and all-or-nothing, so too is the fusion event that releases the sperm's enzymes. This tight control enabled Mayorga's laboratory to capture a molecular movie of fusion as it unfolded. Their findings will be published in the September issue of the journal Public Library of Science Biology.

Inside the sperm, the enzymes are contained in a small bag known as the acrosome. During fertilization, as the acrosome membrane meets the sperm's outer membrane, the two fuse together, and the enzymes are released outside the cell much the same way a bubble rises to the surface of a soda and releases its gas into the air.

Mayorga, who has studied membrane fusion for more than 15 years, recognized an unexplored potential in this simple secretion event, called acrosomal exocytosis (AE). Preliminary experiments showed that AE uses the same basic fusion molecules as neuronal and endocrine cells. However, AE is much less complicated than fusion in these other cell types becau
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Contact: Cindy Fox Aisen
aisenc@hhmi.org
317-843-2276
Howard Hughes Medical Institute
5-Sep-2005

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