HOME >> BIOLOGY >> NEWS
Protein sciences

Dr. Ruth Brack-Werner, Director of the GSF Institute of Molecular Virology (IMV) explains the decisive advantage of the new approach: " In previous assays, signals were generated only by interacting proteins, whereas the individual partners remained undetected. However, the absence of signals could not be used to rule out protein interactions since the absence of one or both interaction partners would have the same effect. To overcome this problem Brack-Werner and her team developed the so-called extended bimolecular fluorescence complementation (exBiFC) which allows simultaneous monitoring of individual proteins and their interactions.

Brack-Werner and her colleagues' groundbreaking research work focusses on mechanisms that control replication of the human immunodeficiency virus (HIV), which causes AIDS. "HIV replication is based on the interaction of cellular proteins with viral proteins. Interactions involving viral regulatory factors have a direct impact on the amount of virus produced by the HIV host cell", Brack-Werner explains. "Preventing HIV proteins from interacting with their crucial partners is a promising approach to developing novel therapies." Therefore the GSF-scientists developed and validated exBiFC with the HIV Rev protein, which is an accelerator of HIV production. Various assays investigating Rev interactions in artificial settings indicate that the activity of Rev depends on the interaction of Rev molecules with each other and with cellular proteins. The latter include Exportin 1, which transports proteins from the nucleus to the cytoplasm and RISP, a modulator of HIV gene expression discovered by the Brack-Werner team in previous studies. Brack-Werner and her team demonstrated that exBIFC allows visualization of interactions of Rev with itself and with Exportin1 and RISP in living cells. In addition they were able to compare the strengths of the interactions of Rev with its partners by analysing the intensities of the signals in
'"/>

Contact: Heinz Joerg Haury
oea@gsf.de
49-893-187-2460
GSF - National Research Center for Environment and Health
8-Mar-2007


Page: 1 2

Related biology news :

1. Protein chatter linked to cancer activation
2. Coming soon -- Protein synthesis without amino acids?
3. Protein pulling -- Learning how proteins fold by pulling them apart
4. Protein interactions targets for Huntington disease therapy
5. Protein enables discovery of quantum effect in photosynthesis
6. Protein found that slows hepatitis C growth in liver cells
7. Protein fragments sequenced in 68 million-year-old Tyrannosaurus rex
8. Protein averts cell suicide but might contribute to cancer
9. Protein structures for the entire yeast proteome
10. Protein overexpression at heart of heart failure
11. Protein identified that regulates effectiveness of Taxol chemotherapy in breast cancer

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/14/2019)... ... August 14, 2019 , ... Carterra® Inc., the ... opened a Customer Experience Center (CEC) and office in Munich, Germany. Rapid European ... The Munich CEC will be a place for researchers throughout Europe to gain ...
(Date:8/14/2019)... , ... August 14, 2019 , ... Representatives with MyBioGate ... and Exhibition Center in Boston. , “We help innovative projects connect with Chinese pharma ... for MyBioGate. , Guo went on to explain that as a part of Biotech ...
(Date:8/14/2019)... , ... August 13, 2019 , ... ... Team, Division of Molecular Pathology, Institute of Cancer Research (ICR) in a ... to learn about the process of biomarker and companion diagnostic development including machine ...
Breaking Biology News(10 mins):
(Date:8/8/2019)... ... August 08, 2019 , ... Inference Solutions , ... organizations, today announced that the company’s Studio Intelligent Virtual Agent (IVA) platform earned ... Inference will officially accept the award at Frost & Sullivan’s annual Excellence in ...
(Date:8/6/2019)... DIEGO (PRWEB) , ... August 06, 2019 , ... ... Marketing Radio (LSMR) have joined forces in launching the biweekly SDBN BUZZ podcast ... further growth. SDBN BUZZ podcast will also serve to promote the region and ...
(Date:8/6/2019)... Ill. (PRWEB) , ... August 05, 2019 , ... ... that Jason Casarella has been recognized as a distinguished leader by PharmaVOICE magazine, ... featured in an article in the magazine’s July/August special issue. , As the ...
(Date:8/4/2019)... ... 2019 , ... Cirtec Medical Corporation, a strategic outsourcing partner for complex medical ... construction on a 30,000 sq. ft. manufacturing facility in the Coyol free zone, ... facility, which is expected to be fully operational by the end of the year, ...
Breaking Biology Technology:
Cached News: