Genome-wide siRNA libraries have the potential to fundamentally change biological research and accelerate drug discovery and development. However, experience with previous breakthrough technologies has shown that intra- and inter-laboratory variations are common at the outset and can limit the ultimate utility of early results. The initial data presented at the second meeting of RNAi Global in Washington, D.C. in April demonstrated that without uniform standards, the results of experiments may not be comparable between laboratories.
"To accelerate generation of meaningful experimental output, twelve members of the RNAi Global Initiative conducted the same experiments using the same protocols in the most extensive multi-site comparative RNAi screen conducted to date," said William S. Marshall, Ph.D., vice president of technology and business development for Fisher Biosciences. "By comparing this large data set we were able to identify several critical areas of potential variation and the key information required for inter-laboratory experimental comparison. Understanding this inherent variation in research results is the principal reason Dharmacon took the unique step of forming a working consortium to develop the experimental parameters necessary to facilitate inter- and intra-laboratory comparisons."
Stefan Wiemann, Ph.D, Group Leader at the German Cancer Research Center (DKFZ) added: "The absence of accepted research standards for the new tool of microarrays in the 1990's cost the life sciences research community many years of prog
Contact: Stephen Gendel