HOME >> BIOLOGY >> NEWS
Researchers use mass spectrometry to detect norovirus particles

Scientists have used mass spectrometry for decades to determine the chemical composition of samples but rarely has it been used to identify viruses, and never in complex environmental samples. Researchers at the Johns Hopkins Bloomberg School of Public Health recently demonstrated that proteomic mass spectrometry has the potential to be applied for this purpose. Using a two-step process, researchers successfully separated, purified and concentrated a norovirus surrogate from a clinical sample within a few hours. Nanospray mass spectrometry was used to demonstrate the feasibility of detecting norovirus particles in the purified concentrates.

Human norovirus is responsible for an estimated 23 million cases of gastrointestinal illness in the United States each year. This pathogen is a particular problem aboard cruise ships. The researchers believe that their mass spectrometric method could potentially be used for biodefense and public health preparedness as a tool for rapidly detecting norovirus--a category B bioterrorism agent--and other viral public health threats. The study is published in the April 2006 edition of Applied and Environmental Microbiology.

In simplified terms, mass spectrometry is essentially a scale for weighing molecules. A laser turns a sample into ionized particles, which are then accelerated in a vacuum toward a detector. The time lapsed prior to registering on the detector helps researchers determine the mass--or weight--of the particles. By targeting characteristic particles, or peptides, belonging to the viral coat protein, the virus can be positively identified by matching the results to entries in genetic databases.

In the Hopkins study, the researchers analyzed a stool sample treated with virus-like particles, which closely resemble norovirus but are noninfectious. Using mass spectrometry, the researchers were able to detect the norovirus capsid protein down to levels typically found in clinical specimens from sic
'"/>

Contact: Tim Parsons or Kenna Lowe
paffairs@jhsph.edu
410-955-6878
Johns Hopkins University Bloomberg School of Public Health
7-Apr-2006


Page: 1 2

Related biology news :

1. Researchers find pathway that controls cell size and division
2. Researchers watch antibiotics, bacteria meet at atomic level
3. Researchers discover gene responsible for Restless Legs Syndrome
4. Researchers witness natural selection at work in dramatic comeback of male butterflies
5. Researchers discover human embryonic stem cells are the ultimate perpetual fuel cell
6. Researchers use new approach to predict protein function
7. Researchers probe risks, benefits of folic acid fortification
8. Researchers identify genetic mutation that may alter tumor cell proliferation
9. Researchers discover method for identifying how cancer evades the immune system
10. Researchers use adult stem cells to create soft tissue
11. Researchers find gene that spurs development of the epididymis

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/8/2017)... , Feb. 7, 2017 Report Highlights ... by 2021 from $8.3 billion in 2016 at a ... to 2021. Report Includes - An overview of ... market trends, with data from 2015 and 2016, and ... - Segmentation of the market on the basis of ...
(Date:2/7/2017)... Feb. 7, 2017 Zimmer Biomet Holdings, Inc. ... healthcare, will present at the LEERINK Partners 6th Annual ... Hotel on Wednesday, February 15, 2017 at 10 a.m. ... the presentation can be accessed at http://wsw.com/webcast/leerink28/zbh .  ... conference via Zimmer Biomet,s Investor Relations website at ...
(Date:2/6/2017)... , Feb. 6, 2017 According to ... are driving border authorities to continue to embrace ... there are 2143 Automated Border Control (ABC) eGates ... deployed at more than 163 ports of entry ... to 2016 achieving a combined CAGR of 37%. ...
Breaking Biology News(10 mins):
(Date:2/24/2017)... ... February 24, 2017 , ... Delpor, Inc. (Delpor), a biotechnology company focused ... Institute of Mental Health (NIMH) for the further advancement of the company’s 3-month olanzapine ... and is expected to deliver therapeutic levels of olanzapine for a period of 3 ...
(Date:2/24/2017)... , Feb. 24, 2017 Symic Bio, a ... a new category of therapeutics, announced today the completion ... in peripheral artery disease. The trial will evaluate the ... therapeutic, in the reduction of restenosis following angioplasty. ... development milestone for SB-030," said Nathan Bachtell , ...
(Date:2/24/2017)... 23, 2017 China Biologic Products, Inc. (NASDAQ: CBPO) ... biopharmaceutical company in China, today announced its financial results for ... Fourth Quarter 2016 Financial Highlights ... 21.7% in RMB terms, or increased by 13.6% in USD ... quarter of 2015. Gross profit increased by ...
(Date:2/23/2017)... 23, 2017 ... per share data, unaudited)Three Months Ended December 31,Twelve Months ... $         ...   89026%Aldurazyme Net Product Revenue ... Net Product Revenue  756025%297303(2)%Vimizim Net ...
Breaking Biology Technology:
Cached News: