HOME >> BIOLOGY >> NEWS
Researchers use mass spectrometry to detect norovirus particles

Scientists have used mass spectrometry for decades to determine the chemical composition of samples but rarely has it been used to identify viruses, and never in complex environmental samples. Researchers at the Johns Hopkins Bloomberg School of Public Health recently demonstrated that proteomic mass spectrometry has the potential to be applied for this purpose. Using a two-step process, researchers successfully separated, purified and concentrated a norovirus surrogate from a clinical sample within a few hours. Nanospray mass spectrometry was used to demonstrate the feasibility of detecting norovirus particles in the purified concentrates.

Human norovirus is responsible for an estimated 23 million cases of gastrointestinal illness in the United States each year. This pathogen is a particular problem aboard cruise ships. The researchers believe that their mass spectrometric method could potentially be used for biodefense and public health preparedness as a tool for rapidly detecting norovirus--a category B bioterrorism agent--and other viral public health threats. The study is published in the April 2006 edition of Applied and Environmental Microbiology.

In simplified terms, mass spectrometry is essentially a scale for weighing molecules. A laser turns a sample into ionized particles, which are then accelerated in a vacuum toward a detector. The time lapsed prior to registering on the detector helps researchers determine the mass--or weight--of the particles. By targeting characteristic particles, or peptides, belonging to the viral coat protein, the virus can be positively identified by matching the results to entries in genetic databases.

In the Hopkins study, the researchers analyzed a stool sample treated with virus-like particles, which closely resemble norovirus but are noninfectious. Using mass spectrometry, the researchers were able to detect the norovirus capsid protein down to levels typically found in clinical specimens from sic
'"/>

Contact: Tim Parsons or Kenna Lowe
paffairs@jhsph.edu
410-955-6878
Johns Hopkins University Bloomberg School of Public Health
7-Apr-2006


Page: 1 2

Related biology news :

1. Researchers find pathway that controls cell size and division
2. Researchers watch antibiotics, bacteria meet at atomic level
3. Researchers discover gene responsible for Restless Legs Syndrome
4. Researchers witness natural selection at work in dramatic comeback of male butterflies
5. Researchers discover human embryonic stem cells are the ultimate perpetual fuel cell
6. Researchers use new approach to predict protein function
7. Researchers probe risks, benefits of folic acid fortification
8. Researchers identify genetic mutation that may alter tumor cell proliferation
9. Researchers discover method for identifying how cancer evades the immune system
10. Researchers use adult stem cells to create soft tissue
11. Researchers find gene that spurs development of the epididymis

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/5/2017)... NEW YORK , April 5, 2017 ... security, is announcing that the server component of the ... is known for providing the end-to-end security architecture that ... customers. HYPR has already secured over 15 ... system makers including manufacturers of connected home product suites ...
(Date:3/30/2017)... -- On April 6-7, 2017, Sequencing.com will host the world,s ... at Microsoft,s headquarters in Redmond, Washington ... health and wellness apps that provide a unique, personalized ... is the first hackathon for personal genomics and the ... the genomics, tech and health industries are sending teams ...
(Date:3/28/2017)... 2017 The report "Video Surveillance ... Servers, Storage Devices), Software (Video Analytics, VMS), and Service ... Forecast to 2022", published by MarketsandMarkets, the market was ... projected to reach USD 75.64 Billion by 2022, at ... base year considered for the study is 2016 and ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... Bay, Florida (PRWEB) , ... October 11, 2017 ... ... and Drug Administration (FDA) has granted orphan drug designation to SBT-100, its novel ... (sdAb) for the treatment of osteosarcoma. SBT-100 is able to cross the cell ...
(Date:10/10/2017)... (PRWEB) , ... October 10, 2017 , ... ... Science Center’s FirstHand program has won a US2020 STEM Mentoring Award. Representatives of ... award for Excellence in Volunteer Experience from US2020. , US2020’s mission is to ...
(Date:10/10/2017)... Calif. , Oct. 10, 2017 SomaGenics ... from the NIH to develop RealSeq®-SC (Single Cell), expected ... for profiling small RNAs (including microRNAs) from single cells ... Program highlights the need to accelerate development of approaches ... "New techniques for measuring levels ...
(Date:10/9/2017)... Oct. 9, 2017  BioTech Holdings announced today ... which its ProCell stem cell therapy prevents limb ... The Company, demonstrated that treatment with ProCell resulted ... saved as compared to standard bone marrow stem ... resulted in reduction of therapeutic effect.  ...
Breaking Biology Technology:
Cached News: