HOME >> BIOLOGY >> NEWS
Researchers use new approach to predict protein function

In a paper published online this month in the journal Nature Chemical Biology, researchers report that they have developed a way to determine the function of some of the hundreds of thousands of proteins for which amino acid sequence data are available, but whose structure and function remain unknown.

The research team, led by University of Illinois biochemistry professor John A. Gerlt, is the first to use a computational approach to accurately predict a proteins function from its amino acid sequence. Their in silico (computer-aided) predictions were validated in the laboratory by means of enzyme assays and X-ray crystallography.

The new approach involved searching databases of known proteins for those with amino acid sequences that had the greatest homology to the unknown proteins. The researchers then used the three-dimensional structures of the most closely matched known proteins in their analyses of protein function.

Using the structural data obtained from this homology modeling, the team performed computerized docking experiments to quickly evaluate whether the unknown proteins were likely to bind to any of a vast library of potential target molecules, or substrates. Determining which substrate binds to a given protein is vital to understanding the proteins function.

This study describes an integrated approach using experimental techniques, computational techniques and X-ray crystallography for predicting the function of a protein of previously unknown function, Gerlt said.

These methods will speed the task of identifying the biological roles of some of the hundreds of thousands of proteins whose functions have not yet been discovered.

Rather than trying to do (laboratory) experiments on 30,000 compounds to determine if they are substrates, with this approach you might do experiments on 10, Gerlt said.

The study involved a family of proteins within the large and diverse enolase superfam
'"/>

Contact: Diana Yates
diya@uiuc.edu
217-333-5802
University of Illinois at Urbana-Champaign
11-Jul-2007


Page: 1 2

Related biology news :

1. Researchers find pathway that controls cell size and division
2. Researchers watch antibiotics, bacteria meet at atomic level
3. Researchers discover gene responsible for Restless Legs Syndrome
4. Researchers witness natural selection at work in dramatic comeback of male butterflies
5. Researchers discover human embryonic stem cells are the ultimate perpetual fuel cell
6. Researchers probe risks, benefits of folic acid fortification
7. Researchers identify genetic mutation that may alter tumor cell proliferation
8. Researchers discover method for identifying how cancer evades the immune system
9. Researchers use adult stem cells to create soft tissue
10. Researchers find gene that spurs development of the epididymis
11. Researchers isolate new risk marker for overweight children

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/6/2019)... ... August 06, 2019 , ... ... with clinical, regulatory and GMP manufacturing experience. In November it spun out ... With extensive regulatory experience and one of the first cGMP cell manufacturing ...
(Date:8/6/2019)... ... August 06, 2019 , ... ... concerns. One plant-based food in particular quickly entered the limelight – meat alternatives. ... use a thermogelling and emulsifying ingredient such as methyl cellulose. This ingredient is ...
(Date:8/1/2019)... ... August 01, 2019 , ... The GENSPEED platform is a ... It is fully developed with over 100 units deployed in the field for ... of use, our collaboration with axiVEND will give us a foothold in the USA ...
Breaking Biology News(10 mins):
(Date:8/6/2019)... ... August 05, 2019 , ... Advanced Clinical, a ... a distinguished leader by PharmaVOICE magazine, earning him a prestigious spot on the ... July/August special issue. , As the executive vice president of business development and ...
(Date:8/4/2019)... ... August 02, 2019 , ... Cirtec Medical Corporation, a strategic outsourcing partner for ... has begun construction on a 30,000 sq. ft. manufacturing facility in the Coyol ... , The facility, which is expected to be fully operational by the end of ...
(Date:7/26/2019)... ... July 25, 2019 , ... LeadingBiotech , an exclusive ... announces its Boston BD conference to be held on September 10, ... Boston , the event provides an opportunity for attendees to leverage insight from ...
(Date:7/19/2019)... , ... July 18, 2019 , ... ... announced their investment to enlarge their current piezo motion facilities in Lederhose, Germany, ... 3 floors for multilayer piezo ceramic assembly production in addition to general office ...
Breaking Biology Technology:
Cached News: