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Scientists prove that disputed Korean stem cell line comes from an unfertilized egg and not cloning

resolved.

The study found that ntES and pES cells have distinct DNA recombination signatures. Those made from parthenogenetic embryos display a telltale genetic pattern close to the center of chromosomes. The results also revealed that the SCNT-hES-1 cell line was not derived by somatic nuclear transfer as was previously claimed. Our analysis shows that the recombination pattern of SCNT-hES-1 is distinct from that of an ntES line and is consistent with its derivation from a parthenogenetic embryo. Thus, we conclude that the derivation of SCNT-hES-1 represented the first successful isolation of human pES cells, offers Dr. Daley.

The authors conclude that, although there are still significant obstacles to overcome in the generation of pES cells, parthenogenesis is an efficient way of generating embryos and that it may someday be feasible to generate patient-specific pES cells from females. If careful genetic and functional analysis of tissues derived from human pES cells show them to be safe and effective, then pES cells might represent a favorable source for tissue replacement therapies, says Dr. Daley.


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Contact: Erin Doonan
edoonan@cell.com
617-397-2802
Cell Press
2-Aug-2007


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