The new study may offer a potential target for an effective, non-hormonal male contraceptive, the researchers said. The findings will be published today (Nov. 15) in the online early edition of the journal Proceedings of the National Academy of Sciences. A report also will appear Nov. 23 in the journal's print edition.

Collaborating with UNC were scientists from the U.S. National Institute of Environmental Health Sciences (NIEHS); Fudan University in Shanghai, China; and the U.S. Environmental Protection Agency.

Sperm motility, produced by the coordinated movement of the extremely long sperm tail, requires substantial energy in the form of adenosine triphosphate, or ATP, the major energy currency of the cell.

Specialized cellular structures known as mitochondria were thought to provide a substantial portion of the ATP needed for sperm motility. In contrast, Dr. Deborah A. O'Brien, associate professor of cell and developmental biology at UNC's School of Medicine, and her colleagues found that sperm motility and ATP production depend primarily on a metabolic pathway known as glycolysis. This pathway uses sugar to produce energy, a common process in animal and plant cells.

The researchers focused on the enzyme glyceraldehyde 3-phosphate dehydrogenase-S, or GAPDS, a novel enzyme in the glycolytic pathway that is expressed only in germ cells very late in the process of sperm production.

GAPDS is tightly bound to a structural element that extends along most of the length of the sperm tail. The study team used gene targeting, or gene knockout technology, to produce mice that could not make this unique enzyme.

Without GAPDS, glycolysis is selectively blocked in sperm and this pathway produces no ATP. As expected, the females were normal and
'"/>

Contact: Leslie H. Lang
llang@med.unc.edu
919-843-9687
University of North Carolina School of Medicine
15-Nov-2004