If researchers could selectively block some of these channels in implanted cells, derived from stem cells, they may be able to prevent potential tumor development. The paper appears Aug. 5 online in the journal Stem Cells.
"A major concern for human ESC-based therapies is the potential for engineered grafts to go haywire after transplantation and form tumors, for instance, due to contamination by only a few undifferentiated human ESCs," says Ronald A. Li, Ph.D., an assistant professor of medicine at The Johns Hopkins University School of Medicine and senior author of the study. "Our discovery of functional ion channels, which are valves in a cell's outer membrane allowing the passage of charged atoms, the basis of electricity, provides an important link to the differentiation, or maturation, and cell proliferation, or growth of human ESCs."
Because human ESCs can potentially provide an unlimited supply of even highly specialized cells, such as brain and heart cells, for transplantation and cell-based therapies, they may provide an ultimate solution to limited donor availability.
In an earlier study, Li's lab genetically engineered heart cells derived from human ESCs, suggesting the possibility of transplanting unlimited supplies of healthy, specialized cells into damaged organs.
"We do not want to be taking any chances of tumor formation. Based on our previous research, we therefore decided to explore the existence of ion channels in pluripotent, or versatile, human ESCs because electrical activity is known to regulate cell differentiation and proliferation," says Li. "To my knowledge, the electrical properties of
Contact: Joanna Downer
Johns Hopkins Medical Institutions