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Stem cells get a grip

In real estate, its location, location, location. In stem cells, its niche, niche, niche.

Stems cells are regulated by "intrinsic factors" but its also becoming increasingly clear that their "stemness" depends on the unique microenvironment called the stem cell niche.

No adult or somatic stem cells have a more complex niche life than hematopoietic stem cells, which give rise to all the different types of blood and immune cells. The bone marrow is their niche. Its a crowded neighborhood, teeming with different cell types such as fibroblasts, adipocytes, macrophages, and endothelial cells. Hematopoietic stem cells also share their bone marrow niche with osteoblasts, the bodys bone-forming cells, which are derived from a different stem cell population called bone marrow stromal cells. The two stem cells lines probably have a common progenitor but for now scientists can only regard hematopoietic stem cells and osteoblasts as niche neighbors. But its a very close relationship, as researchers in the National Institute of Child Health & Human Development laboratory of Jennifer Lippincott-Schwartz now reveal in great detail.

Earlier reports had established that osteoblasts are a crucial component of the hematopoietic stem cell niche and that physical contact with osteoblasts was vital for their survival. The Lippincott-Schwartz lab used live cell imaging techniques to see what happens when the two cell types are put together, directing their attention in particular at the point of cell-cell contact, and how it affects the cellular and molecular relationship of the two cell types.

The NIH researchers observed dynamic amoeboid motility toward the osteoblasts by both human CD34+ hematopoietic progenitor cells and KG1 cells, which are a hematopoietic subset of myeloid progenitors. The progenitor cells assumed a polarized morphology with a leading edge structure and a foot-like "special domain" projection from the cells trailing edg
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Contact: John Fleischman
jfleischman@ascb.org
513-929-4635
American Society for Cell Biology
14-Dec-2006


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