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Study urges caution in contaminant source tracking

he country.

"All too often, results of these analyses are offered without enough validation of accuracy," Stoeckel explained. "Source tracking study results should always be supported by quality control data. Interpretation of the results can be supported by multiple lines of evidence, such as land use data and presence of wastewater chemicals such as caffeine, when pointing to sources of contamination. Both the client and the analyzing laboratory have a part in making sure interpretations are accurate."

Prior source tracking research reports cite accuracy ranges from 6090 percent for various source tracking methods. The authors of the USGS study attribute the discrepancy between the 6090 accuracy rates and the 2030 accuracy rates they reported to a number of other factors:

  • Different bacteria may be present in animal guts in different seasons; in the USGS study, challenge isolates were collected 9 months after the reference feces were collected;

  • There may be too many strains of E. coli bacteria in each animal species for effective application with small reference libraries, such as the 900 reference strains in the USGS study. At a cost of $10 to $100 to analyze one reference strain, however, building large source libraries gets expensive rather quickly.

  • E. coli strains may not be truly specific to one animal source. Some E. coli strains have been found in more than one animal source, such as when animals live in close proximity with one another, though no evidence to support this premise was found in the USGS study.

    Further research may lead to improvements in current source tracking methods or development of better methods. For the immediate future, researchers and end users would be prudent to use caution and to incorporate quality-control measures to validate the accuracy of source tracking results.


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  • 6-Dec-2004


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