Super small nanoelectrodes can probe microscale environments

CHAMPAIGN, Ill. -- Investigating the composition and behavior of microscale environments, including those within living cells, could become easier and more precise with nanoelectrodes being developed at the University of Illinois.

"The individual nanotube-based probes can be used for electrochemical and biochemical sensing," said Min-Feng Yu, a U. of I. professor of mechanical science and engineering, and a researcher at the universitys Beckman Institute. "The position of the nanoelectrodes can be controlled very accurately."

To fabricate the nanoelectrodes, Yu and graduate students Kyungsuk Yum, Jie Hu and Han Na Cho begin by attaching a strong, rigid, boron-nitride nanotube to a much larger, conductive probe. The nanotube will form the insulating core of the nanoelectrode.

The researchers then coat the nanotube with a thin film of gold about 10-50 nanometers thick (a nanometer is 1 billionth of a meter.) The gold layer is then coated with an insulating polymer coating about 10 nanometers thick. Lastly, the researchers use a focused ion beam to slice off the end of the nanotube, exposing a conducting ring of gold sandwiched between an insulating core and an insulating outer ring.

The process yields nanoelectrodes with a diameter of 100 nanometers, and a length of up to 30 microns.

Because the nanotube is attached to a much larger probe, the researchers can manipulate the nanotube like a needle. They can control precisely where the nanotube penetrates a cell, for example, and even pinpoint smaller cell structures, such as the nucleus or mitochondrion.

"Nanoelectrodes offer new opportunities for electrochemical sensing in intracellular environments," said Yu, who will describe the fabrication process and demonstrate the feasibility of nanoelectrodes at the March meeting of the American Physical Society, to be held in Denver, March 5-9. "By functionalizing the active area of the nanoelectrode with an appropriate chemical, we can target

Contact: James E. Kloeppel
University of Illinois at Urbana-Champaign

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