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System drastically cuts down botulism detection time

Washington, D.C. -- One of the most lethal substances in the world -- botulism -- can be detected using special systems in about 2025 minutes. Now, researchers at DOE's Pacific Northwest National Laboratory can detect its presence in five minutes, using the lab's successful Biodetection Enabling Analyte Delivery System, or BEADS combined with optical detection.

The detection system includes three critical components. First, BEADS isolates the toxin from environmental samples. Next, an antibody is used to purify and concentrate the pathogen or toxin to enable accurate and sensitive detection. Finally, a second antibody, called a reporter antibody, labeled with a fluorescent dye or a fluorescent quantum dot binds to a different region on the toxin or pathogen. The fluorescence of the quantum dot is measured on the bead and can quantify the concentration of the toxin.

The BEADS system has been commercially licensed to a few companies. In addition to detecting botulinum, the system can be tailored to detect multiple pathogens or toxins, such as E. coli, salmonella and ricin, simultaneously.


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Contact: Greg Koller
greg.koller@pnl.gov
509-372-4864
DOE/Pacific Northwest National Laboratory
1-Sep-2005


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