HOME >> BIOLOGY >> NEWS
UW-Madison engineers squeeze secrets from proteins

Proteins, one of the basic components of living things, are among the most studied molecules in biochemistry. Understanding how proteins form or "fold" from sequenced strings of amino acids has long been one of the grand challenges of biology.

A common belief holds that the more proteins are confined by their environment, the more stable - or less likely to unfold - they become. Now, as reported on the cover of the March issue of Biophysical Journal, a team of chemical and biological engineers from UW-Madison shows that premise to be untrue. While confinement plays an important role, other factors are also at play.

"Most research in this area looked at proteins in free solution when in fact, most proteins are confined in some way," says Juan de Pablo, a chemical and biological engineer at the University of Wisconsin-Madison. "What we demonstrate for the first time is that the stability of proteins under severe confinement, which is really the relevant way of looking at them for numerous applications, depends on their shape, their size and their interactions with the environment. It is a delicate balance between the energy available to fold the protein and entropy, or it's desire to be in the unfolded state."

De Pablo's research team developed a method to precisely calculate the entropy and determine how much of a protein's stability change upon confinement to attribute to energy and how much to entropy. "This is the important part of the calculation," de Pablo adds.

Protein stability is an incredibly important property in myriad applications, de Pablo says. Consider laundry detergent. A popular ad for detergent once claimed that "protein gets out protein." The idea behind this is that engineered enzymes are at work in the wash breaking down elements of a stain.

"Once a protein is folded, you can actually unfold it or destabilize it, either by heating it up, or by adding solvents to the system, like urea for example, that j
'"/>

Contact: Juan de Pablo
depablo@engr.wisc.edu
608-516-7877
University of Wisconsin-Madison
21-Mar-2006


Page: 1 2

Related biology news :

1. UW-Madison work on stem cells, cardiac health to be presented at ACS
2. UW-Madison researchers find new way to sweeten key drugs
3. UW-Madison research part of international mercury conference
4. UW-Madison small-scale research receives big boost
5. $3.4 million directed to key UW-Madison MS study
6. UW-Madison scientists zero in on drugs sweet spots
7. UW-Madison gains two new stem cell programs
8. Water research initiatives at UW-Madison
9. Boston Univeristy bioengineers devise dimmer swith to regulate gene expression in mammal cells
10. Worlds top engineers in San Antonio for system of systems conference
11. New medications, cancer diagnosis goals of UH engineers with $1M in grants

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/21/2016)... 21, 2016 NuData Security announced today that ... of principal product architect and that Jon ... customer development. Both will report directly to ... moves reflect NuData,s strategic growth in its product ... customer demand and customer focus values. ...
(Date:6/15/2016)... ALBANY, New York , June 15, 2016 ... published a new market report titled "Gesture Recognition Market ... Trends and Forecast, 2016 - 2024". According to the ... at USD 11.60 billion in 2015 and is ... and reach USD 48.56 billion by 2024.  ...
(Date:6/7/2016)... , June 7, 2016  Syngrafii Inc. and ... business relationship that includes integrating Syngrafii,s patented LongPen™ ... project. This collaboration will result in greater convenience ... credit union, while maintaining existing document workflow and ... ...
Breaking Biology News(10 mins):
(Date:11/30/2016)... , November 30, 2016 The global ... few players hold a dominant share in the overall ... Laboratories International, Inc., and Merck KGaA, held a lion,s ... Transparency Market Research observes that these companies are expected ... development products that are do not require rabbit pyrogen ...
(Date:11/30/2016)... VANCOUVER , Nov. 30, 2016 /PRNewswire/ -  Equicare ... coordination solutions, has been recognized as one of the ... 100, an annual international listing that distinguishes the top ... "We,ve pushed a great step forward this year continually ... growing our own customer base and team," says ...
(Date:11/30/2016)... -- Part of 5m$ Investment in Integrated Drug ... , ... today announced that it had successfully completed the expansion of ... increased the Screening Collection to over 400,000. The new compounds ... the company. This expansion, complemented by new robotics and compound ...
(Date:11/30/2016)... Triangle Park, NC (PRWEB) , ... November 30, ... ... development company engaged in the development of a new orally administered treatment for ... testing and neuroimaging results of a Phase 2a clinical trial of T3D-959 in ...
Breaking Biology Technology:
Cached News: