Unlocking proteins from their cellular shell

PHILADELPHIA Applying physical stress to cells, researchers at the University of Pennsylvania have demonstrated that everyday forces can alter the structure of proteins tucked within cells, unfold them and expose new targets in the fight against disease.

The findings for both simple red blood cells and more versatile stem cells show that hidden and folded parts of proteins can be exposed by physical strain, not just by chemical reaction. From there, proteins can be labeled and mapped, increasing the understanding of cellular behavior and unlocking novel targetable sites for drugs that might interact with these active proteins to treat disease.

The study appears in the current issue of the journal Science.

Cells are exposed to forces every minute of every day. Some forces are exerted by the flow of bodily fluids, such as blood streaming through arteries, while other forces are generated by cells as they crawl and contract in tissue. Penn researchers used force to induce changes in the structures of cytoskeletal proteins and, employing fluorescent dyes, labeled proteins sequentially, visualized them, and molecularly pinpointed the newly exposed parts of proteins. By forcing proteins to unfold, hidden binding sites were thus revealed. Multicolor maps were generated that revealed locations of protein sites not accessible when the cell remained static and relaxed.

"We were motivated to probe molecular mechanics within cells in part by our recent findings on stem cells that show they generate their own forces, switching on and off development of different cell types," Dennis Discher, professor of chemical and biomolecular engineering at Penn, said.

Proteins are the molecular instigators for almost every biologic action and reaction in living things. Their assembly and reactions are the foundation of processes from the division of cells to the complex chain of events that result in the apparently simple blink of an eye. Path

Contact: Jordan Reese
University of Pennsylvania

Page: 1 2

Related biology news :

1. Unlocking mysteries of brain, grooming future med students goals of UH prof
2. Unlocking genetic mysteries focus of UH symposium
3. UCF research links proteins, stem cells and potential Alzheimers treatment
4. Teamwork between 2 key proteins necessary for normal development and regulation of red blood cells
5. Call for EU to launch major project to map out all our proteins
6. MIT IDs link between brain tumor proteins
7. Protein pulling -- Learning how proteins fold by pulling them apart
8. Measuring the unseeable: Penn researchers probe proteins dark energy
9. New use for a cell toxin found to inhibit survival proteins in cancer cells
10. Pairing nanoparticles with proteins
11. Targeting key proteins of carcinogenesis

Post Your Comments:

(Date:3/27/2017)... ROCKVILLE CENTRE, N.Y. , March 27, 2017 ... by Healthcare Information and Management Systems Society (HIMSS) ... Analytics Outpatient EMR Adoption Model sm . In ... top 12% of U.S. hospitals using an electronic ... recognized CHS for its high level of EMR ...
(Date:3/24/2017)... Research and Markets has announced the addition of the ... Forecast to 2025" report to their offering. ... The Global Biometric Vehicle Access System ... over the next decade to reach approximately $1,580 million by 2025. ... forecasts for all the given segments on global as well as ...
(Date:3/23/2017)... , Mar. 23, 2017 Research and ... System Market Analysis & Trends - Industry Forecast to 2025" ... ... grow at a CAGR of around 8.8% over the next decade ... industry report analyzes the market estimates and forecasts for all the ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... SARASOTA, FL (PRWEB) , ... ... ... Inc. (RPS®) today announces publication of a United States multicenter, prospective clinical ... single use, disposable, point-of-care diagnostic test capable of identifying clinically significant acute ...
(Date:10/11/2017)... , ... October 11, 2017 , ... ... gene in its endogenous context, enabling overexpression experiments and avoiding the use of ... small RNA guides is transformative for performing systematic gain-of-function studies. , This ...
(Date:10/11/2017)... Netherlands and LAGUNA HILLS, Calif. ... Institute of Cancer Research, London (ICR) ... MMprofiler™ with SKY92, SkylineDx,s prognostic tool to risk-stratify patients with ... known as MUK nine . The University of ... which is partly funded by Myeloma UK, and ICR will ...
(Date:10/10/2017)... (PRWEB) , ... October 10, ... ... development-stage cancer-focused pharmaceutical company advancing targeted antibody-drug conjugate (ADC) therapeutics, today confirmed ... targeted HPLN (Hybrid Polymerized Liposomal Nanoparticle), a technology developed in collaboration with ...
Breaking Biology Technology:
Cached News: