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Antibodies for Studying NMDA Receptor Protein Expression and,,,Synapse-Specific Immunolabeling

All three of these antibodies are suitable for Western blot, immunohistochemistry and immunoprecipitation analyses. For added convenience, Stratagene offers these antibodies with specific positive controls. Anti-NMDAR2A and Anti-NMDAR2B include a vial of rat hippocampus homogenate; Anti-NMDAR2C includes a vial of rat cerebellum homogenate.

Synapsin Antibody

figure 3

Synapsin I is a neuron-specific protein that localizes the presynaptic terminal where it associates exclusively with small synaptic vesicles. Immunolabeling of synapsin provides an ideal marker for the presynaptic terminal; previously, it has been used as a marker for synapse formation and elimination.8 Stratagene introduces an affinity-purified rabbit polyclonal synapsin antibody, Anti-Synapsin I, that can be used for studying synapsin expression in different cell types and brain regions. This antibody is suitable for studies of synapsin expression in a variety of different species, including human,9 rat,10 frog and squid.11 The specific immunoreactivity of Anti-Synapsin I is shown (figure 3). Anti-Synapsin I is useful as a synapse-specific marker that can be used to monitor synapse number and synaptogenesis. Again, Stratagene offers Anti-Synapsin I with a purified synapsin positive control, a great convenience for research applications.

Conclusions

Antibodies raised against specific peptides or fusion proteins can be used to specifically target receptor protein subunits and to localize, quantify and characterize receptor protein subunits in tissues and cell lines. Stratagene now offers sequence-specific antibodies against NR2A, NR2
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