( ults were readily detected (...)Simple, Sensitive, and Rapid Detection of FLAG -Tagged,,,Fusion Proteins

ults were readily detected (Figure 4, strip #4). Thus, the TagDetect FLAG Protein Dipstick test strips can be used to monitor FLAG fusion gene expression and follow fusion proteins through purification steps and further analysis.

Figure 4

We carried out specificity studies by applying samples of nonrelevant cell extracts and purified proteins onto the membrane in the same manner as the positive samples. These samples were prepared from E. coli, yeast, and mammalian cells. The sample quantities and test procedures used were identical to the positive samples. At the end of the experimental time frame, the samples tested negative (Figure 4, strips #5 to #11).

Detecting FLAG Fusion Proteins in Mammalian Cell Lysates

To demonstrate how the TagDetect system works to monitor expression and purification, we used test strips to detect FLAG fusion proteins in mammalian cell lysates. Unlike the E. coli and the yeast samples described above, relative concentrations of the FLAG fusion protein are much lower in samples derived from mammalian epitope tagging.³ As shown in Figure 3, we detected a FLAG-luciferase fusion protein expressed from the CMV promoter in CHO cell lysates (strip #9) but did not observe them in the nonexpressed and nontagged proteins (strips #10 and #11). Thus, the kit provides a quick assay for FLAG fusion proteins before a full experimental protocol can be performed.

Conclusions

Stratagenes TagDetect assay kit detects and monitors FLAG-tagged fusion protein expression in yeast, E. coli, and mammalian cells. Its easy, unique technique speeds up detection, so the fastest possible results are realized,
'"/>

Source:

Page: All 1 2 3 4
Related biology technology :

1. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
2. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
3. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
7. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
8. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
9. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
10. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
11. Rapid hit confirmation and early hERG liability testing using the IonWorks HT system