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Carnegie Mellon uses AFM to discover effects of experimental drugs on Alzheimer's plaques

r beam reflected off the lever's end monitors its vertical motion. Perturbation of the lever motion by nanoscale features of a sample's surface topography is used to reconstruct a detailed three-dimensional map of the surface. In the study, the research team used custom-written software to process and to analyze their AFM images.

AFM is an appealing tool, according to Kowalewski, because it provides three-dimensional nanoscale images and, unlike some other techniques, it does not require specialized preparation or contrasting agents that could introduce artifacts. In addition, it allows to study the samples in liquids, under nearly physiological conditions, according to Kowalewski, who is now pursuing this kind of AFM studies to understand the interaction of antibodies and lipoproteins, with the A peptide.

Collaborating investigators include Dan L. Czilli, Bruce Gitter and Ronald B. DeMattos (Eli Lilly and Company) and David M. Holtzman (Washington University).


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Contact: Lauren Ward
wardle@andrew.cmu.edu
412-268-7761
Carnegie Mellon University
20-Jan-2004


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