HOME >> MEDICINE >> NEWS
Diagnosis of prions in patients should utilize novel strategy, team says

ensitivity of the CDI suggests that CDI-like tests could also prove useful for diagnosing other neurodegenerative diseases, such as Alzheimer's disease, Parkinsons's disease and fronto-temporal dementias, all of which, like prion diseases, involve various forms of protein misprocessing. These diseases currently are diagnosed by neuropathological analysis and immunohistochemistry.

"Whether immunohistochemistry underestimates the incidence of one or more of these common neurodegenerative diseases is unknown, but the CDI could shed light on these diseases," says co-author Bruce Miller, MD, UCSF A.W. and Mary Margaret Clausen Distinguished Professor of Neurology and director of the UCSF Memory and Aging Center.

The finding will be printed on-line and in print on March 1, 2005 in Proceedings of the National Academy of Sciences.

The study brings into high relief the different detection strategies of immunohistochemistry and the CDI, both of which involve revealing the presence of prions, known as PrPsc, by applying antibodies to brain tissue.

Standard immunohistochemistry, developed in the DeArmond lab 20 years ago, involves using an enzyme known as a protease, or a combination of harsh acid and high temperature treatment, to destroy normal prion protein (PrPC), which is ubiquitous in brain tissue. Once this occurs, scientists apply fluorescently lit antibodies that react with residues of the relatively resistant abnormal prion protein (PrPSc), thereby highlighting it.

The limitation of this technique is that scientists have since learned that there is a large part of the abnormal prion protein that is protease sensitive, and that portion escapes detection by the standard technique. Thus, this traditional method underestimates the level of PrPSc in tissue.

The CDI addresses this limitation by revealing the region of PrPSc that is exposed in the normal PrPC but is buried in infectious PrPSc, using high affinity, newly
'"/>

Contact: Jennifer O'Brien
jobrien@pubaff.ucsf.edu
415-476-2557
University of California - San Francisco
21-Feb-2005


Page: 1 2 3 4

Related medicine news :

1. Diagnosis: mismanagement
2. Cedars-Sinai Medical Centers Prenatal Program Pushes the Frontiers of Diagnosis From the Second Trimester Into the First
3. New Survey Finds Physicians Seek More Clarity In MS Diagnosis And Management
4. New study shows how mad cow prions hitch a ride into intestine
5. Hormonal treatment improves survival in high-risk prostate cancer patients
6. Study finds that coordinating care of chronically ill patients does not increase liability
7. Mouse model reveals potential way to reduce cardiac deaths in kidney patients
8. Optical technique identifies vulnerable plaques in cardiac patients
9. NHS target driven culture is failing patients
10. Cardiac deaths peak in sleep hours for patients with sleep apnea
11. Chronic fatigue patients show lower response to placebos

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/7/2016)... VA (PRWEB) , ... December 07, 2016 , ... ... are celebrating the passage of the most comprehensive mental health systems reform legislation ... the support of the President, and the commitment of our elected officials to ...
(Date:12/7/2016)... ... December 07, 2016 , ... OC87 Recovery Diaries ... schizophrenic mother in a unique, personal perspective through animation. , That woman ... addictive disorders at her private psychotherapy practice. Sheri’s mother, Pearl, lived with schizophrenia. ...
(Date:12/7/2016)... CT (PRWEB) , ... December 07, 2016 , ... A ... prompted an outpouring of comments on social media that offer a rare glimpse into ... psychiatrist, leading ADHD authority and New York Times bestselling author, has described people with ...
(Date:12/7/2016)... ... December 07, 2016 , ... The Cliff Hart Agencies, a ... Lyon, Dewitt, Williamston, East Lansing, Ann Arbor, and Farmington Hills, is announcing a ... brain tumor. , Jason Bauer and his family are longtime members of the ...
(Date:12/7/2016)... NY (PRWEB) , ... December 07, 2016 , ... A. ... business owners and families throughout the Five Boroughs, is launching a charity drive to ... works to continue the traditions and spirit of marines and Navy FMF Corpsmen. Working ...
Breaking Medicine News(10 mins):
(Date:12/7/2016)... Mich. , Dec. 7, 2016 A new ... readied for human clinical trials in search of the world,s ... following publication of research findings today in the journal ... hope this will be a watershed moment for millions of ... Ph.D. , director of Van Andel Research Institute,s ...
(Date:12/7/2016)... , Dec. 7, 2016 REPORT OBJECTIVES ... Intelligence (2011-2020)" provides market intelligence on a market ... (2011-2020) has been provided in the report. The ... 1) comprehensive global market intelligence through detailed segmentation, ... dynamics, industry structure and developments, market situation, trends, ...
(Date:12/7/2016)... BETHESDA, Md. , Dec. 7, 2016 /PRNewswire/ ... Bio" or the "Company"), a biotechnology company developing ... today announced that the Nasdaq Staff has not ... violations of Listing Rules previously reported, and the ... voluntarily withdraw the Company,s common stock from listing ...
Breaking Medicine Technology:
Cached News: