HOME >> MEDICINE >> NEWS
Myosin molecule walks like a person, experiment shows

n and his Illinois colleagues -- physics professor Taekjip Ha and graduate students Ahmet Yildiz and Sean McKinney -- developed a single-molecule imaging technique that is capable of locating the position of a fluorescent dye to within 1.5 nanometers. (One nanometer is a billionth of a meter, or about 10,000 times smaller than the width of a human hair). This localization represents a 20-fold improvement over other techniques that use fluorescent dyes.

The researchers also found a way to extend the lifetime of the dye from a few seconds to several minutes. Then they teamed up with physiology professor Yale Goldman and postdoctoral researcher Joseph Forkey, both at the University of Pennsylvania, in applying the technique to measuring myosin movement.

"First, we attach a little fluorescent dye to one of the feet and we take a picture with a digital camera attached to a microscope to find exactly where the dye is," Selvin said. "Then we feed the myosin a little food called adenosine triphosphate, and it takes a step. We take another picture, locate the dye, and accurately measure how far the dye moved."

By looking at the step size, the scientists can tell whether the protein is walking or inchworming along, Selvin said. If it is walking, the rear foot takes twice as big a step than if it were inchworming.

"The long dye lifetime allowed us to measure many consecutive steps, which occurred about once every 3 seconds," Selvin said. "The foot wearing the dye would move forward 74 nanometers, then pause while the unlabeled foot moved forward. The cycle would then repeat itself. The 74-nanometer step size we measured is consistent with a hand-over-hand walking mechanism and inconsistent with an inchworm mechanism."

As an additional check, the researchers also labeled the myosin higher up on the leg, "somewhere in the neighborhood of the thighbone," Selvin said. "The steps alternated in size between long and short, just as yo
'"/>

Contact: James E. Kloeppel, Physical Sciences Editor
kloeppel@uiuc.edu
217-244-1073
University of Illinois at Urbana-Champaign
5-Jun-2003


Page: 1 2 3

Related medicine news :

1. Myosin mutant points to human origins
2. Medical molecules designed to respond to visible light that can penetrate tissue
3. Chemists synthesize molecule that helps body battle cancers, malaria
4. Blocking molecules protects health of implants
5. ASU researchers demonstrate new nanotech effect - moving water molecules by light
6. Zengen reports positive phase I/II trial results of its peptide molecule for vaginal yeast infection
7. New molecule may be basis for drugs that battle overeating and drug dependency
8. Latest cancer research features small molecule therapy, and the preventative benefits of sunshine
9. Student builds micro biosensor chip to move DNA molecules
10. Laser method identifies, counts toxic molecules
11. Vision-producing cells fail taste-test, treat key light-detecting molecules identically

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/13/2017)... ... October 13, 2017 , ... ProVest Insurance Group, a ... Miami, and Raleigh regions, is organizing an extended charity drive to benefit the ... chromosome abnormality. , After struggling since birth with several health challenges, T.J. was ...
(Date:10/13/2017)... ... ... As health professionals work to improve their approach to healthcare, there is ... than filling out a survey; in many cases health professionals and patients are working ... and research on the importance of active engagement with patients and members of the ...
(Date:10/13/2017)... ... ... The American Board of Family Medicine's (ABFM) Board of Directors has selected Warren ... James C. Puffer upon his retirement. Dr. Newton will serve in the position of ... end of 2018. Upon assuming the role of President and CEO on January 1, ...
(Date:10/13/2017)... Las Vegas, Nevada (PRWEB) , ... October 13, 2017 , ... ... of 7® Hemp CBD Oil utilizing Purzorb™ technology. Applying the Purzorb™process to full spectrum ... CBD dose required and providing a CBD form that can be easily incorporated into ...
(Date:10/13/2017)... ... ... Lori R. Somekh, founder of the Law Office of Somekh & ... and special needs planning attorneys. “Membership in ElderCounsel helps our office remain up to ... with elder law attorneys nationwide,” said Somekh. , ElderCounsel was founded ...
Breaking Medicine News(10 mins):
(Date:9/22/2017)... 2017 AVACEN Medical (AVACEN) announced that its ... helping those with the widespread pain associated with fibromyalgia ... in Essex, England commented, "I ... experiencing no sleep at all, tremendous pain, with every ... recommend [the AVACEN 100] enough, how this has and ...
(Date:9/18/2017)... , Sept. 18, 2017 EpiVax, ... of bioinformatics and immune engineering, today announced ... influenza A (H7N9) vaccine. ... to seasonal influenza and presents a challenge ... prior exposure to be effective. Using state-of-the-art ...
(Date:9/12/2017)... 12, 2017  ValGenesis Inc., the global leader ... pleased to announce the appointment of Dr. ... Board of Directors and Chairman of Advisory Board ... science companies to manage their entire validation lifecycle ... in this process. Furthermore, ValGenesis VLMS enables rigorous ...
Breaking Medicine Technology:
Cached News: