HOME >> MEDICINE >> NEWS
Myosin molecule walks like a person, experiment shows

n and his Illinois colleagues -- physics professor Taekjip Ha and graduate students Ahmet Yildiz and Sean McKinney -- developed a single-molecule imaging technique that is capable of locating the position of a fluorescent dye to within 1.5 nanometers. (One nanometer is a billionth of a meter, or about 10,000 times smaller than the width of a human hair). This localization represents a 20-fold improvement over other techniques that use fluorescent dyes.

The researchers also found a way to extend the lifetime of the dye from a few seconds to several minutes. Then they teamed up with physiology professor Yale Goldman and postdoctoral researcher Joseph Forkey, both at the University of Pennsylvania, in applying the technique to measuring myosin movement.

"First, we attach a little fluorescent dye to one of the feet and we take a picture with a digital camera attached to a microscope to find exactly where the dye is," Selvin said. "Then we feed the myosin a little food called adenosine triphosphate, and it takes a step. We take another picture, locate the dye, and accurately measure how far the dye moved."

By looking at the step size, the scientists can tell whether the protein is walking or inchworming along, Selvin said. If it is walking, the rear foot takes twice as big a step than if it were inchworming.

"The long dye lifetime allowed us to measure many consecutive steps, which occurred about once every 3 seconds," Selvin said. "The foot wearing the dye would move forward 74 nanometers, then pause while the unlabeled foot moved forward. The cycle would then repeat itself. The 74-nanometer step size we measured is consistent with a hand-over-hand walking mechanism and inconsistent with an inchworm mechanism."

As an additional check, the researchers also labeled the myosin higher up on the leg, "somewhere in the neighborhood of the thighbone," Selvin said. "The steps alternated in size between long and short, just as yo
'"/>

Contact: James E. Kloeppel, Physical Sciences Editor
kloeppel@uiuc.edu
217-244-1073
University of Illinois at Urbana-Champaign
5-Jun-2003


Page: 1 2 3

Related medicine news :

1. Myosin mutant points to human origins
2. Medical molecules designed to respond to visible light that can penetrate tissue
3. Chemists synthesize molecule that helps body battle cancers, malaria
4. Blocking molecules protects health of implants
5. ASU researchers demonstrate new nanotech effect - moving water molecules by light
6. Zengen reports positive phase I/II trial results of its peptide molecule for vaginal yeast infection
7. New molecule may be basis for drugs that battle overeating and drug dependency
8. Latest cancer research features small molecule therapy, and the preventative benefits of sunshine
9. Student builds micro biosensor chip to move DNA molecules
10. Laser method identifies, counts toxic molecules
11. Vision-producing cells fail taste-test, treat key light-detecting molecules identically

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Myosin molecule walks like person experiment shows

(Date:7/29/2015)... ... , ... This year, the threat of flooding is higher in many areas ... water, mold is not far behind. Paul Davis, a leading provider of property restoration ... damage. Paul Davis is regularly called on to handle these types of emergencies, although ...
(Date:7/29/2015)... ... July 29, 2015 , ... CrossFit 269, offering a nationally ... Kalamazoo Strength & Conditioning. , The name change coincides with the release of ... training center over the past three years,” according to Jack Kelly, the center’s ...
(Date:7/29/2015)... ... July 29, 2015 , ... Orthopedic surgeons at Lourdes Medical Center of ... Rotator Cuff System, a new implant to treat a common source of shoulder pain ... regeneration of additional tendon tissue, which could allow for faster healing and a decreased ...
(Date:7/29/2015)... ... July 29, 2015 , ... Wedding season is in full swing, which means bridal beauty ... of looking good on the big day. Every bride wants to look and feel beautiful, ... , According to a recent study by the American Academy of ...
(Date:7/29/2015)... ... July 29, 2015 , ... Fastline Publications, ... of 2015 on RFD-TV. , Fastline is dedicated to quality agricultural advertising, both ... contains the largest and freshest searchable agriculture equipment database in the world. The ...
Breaking Medicine News(10 mins):Health News:Paul Davis Offers Tips to Minimize Property Damage and Health Risks This Flood Season 2Health News:Paul Davis Offers Tips to Minimize Property Damage and Health Risks This Flood Season 3Health News:Kalamazoo’s CrossFit 269 Changes Name, Opens New Website 2Health News:Kalamazoo’s CrossFit 269 Changes Name, Opens New Website 3Health News:Kalamazoo’s CrossFit 269 Changes Name, Opens New Website 4Health News:Lourdes Orthopedics First in NJ to Offer Bioinductive Implant for Rotator Cuff Repair 2Health News:Lourdes Orthopedics First in NJ to Offer Bioinductive Implant for Rotator Cuff Repair 3Health News:Say I Do To Surgery: Bridal Beauty On The Rise According to New Survey 2Health News:Say I Do To Surgery: Bridal Beauty On The Rise According to New Survey 3Health News:Fastline Publications to Partner with American Farmer in Upcoming Episode Airing on RFD-TV 2
(Date:7/29/2015)... Calif. , July 29, 2015 /PRNewswire/ ... or the "Company"), the leading provider of ... sciences industry, serving markets including immunotherapies, stem ... manufacturers, animal health, and reproductive medicine, announced ... sale of common stock and warrants (the "Units") under ...
(Date:7/29/2015)... 2015 Research and Markets ( http://www.researchandmarkets.com/research/sxwk89/3d_metrology ) ... Market by Product (CMM, ODS, and VMM), Application (Automotive, ... (North America, Europe, APAC, RoW) - Global Forecast to ... The 3D metrology market was valued at $1,456.7 ... a rate of 7.11% during the forecast period. ...
(Date:7/29/2015)... DIEGO , July 29, 2015  ViaCyte, Inc., a privately-held ... for the treatment of diabetes in clinical trials, today announced the ... STEP ONE, or S afety, T olerability, and ... One Diabetes.  The new site, which is the first in ... Alberta Hospitals in Edmonton , Alberta.  ...
Breaking Medicine Technology:Cryoport Completes $6.8 Million Equity Capital Raise 2Cryoport Completes $6.8 Million Equity Capital Raise 3Global 3D Metrology Market 2015-2020: Products (CMM, ODS, and VMM) & Applications (Automotive, Aerospace, Energy & Power, Medical and Electronics) 2ViaCyte Announces Second Clinical Trial Site at University of Alberta 2ViaCyte Announces Second Clinical Trial Site at University of Alberta 3ViaCyte Announces Second Clinical Trial Site at University of Alberta 4
Cached News: