HOME >> MEDICINE >> NEWS
New quick test for drug-resistant strains of gonorrhea

Potential antimicrobial resistance in the bacteria that cause gonorrhea can be detected without culturing the organism, thanks to a rapid test developed by researchers at Johns Hopkins.

Key to the usefulness of the new test is that it does not require collection, culture or testing of the bacteria themselves - called Neisseria gonorrhea. Instead, the genes linked to resistance can be identified in urine samples or in leftover products from other commonly used diagnostic techniques, the Hopkins team reports.

This new application of probe technology should help public health officials study the spread of antibiotic-resistant gonorrhea by simplifying analysis of samples that cannot be used for culturing organisms, the Hopkins group added.

The Hopkins team developed the test by using an existing diagnostic technology called nucleic acid amplification tests (NAATs). These are FDA-approved tests that detect gonorrhea DNA in urine samples. Using leftover DNA from NAATs, the team performed a polymerase chain reaction (PCR) to make copies of genes linked to resistance. They then performed a melt curve analysis to detect mutations in these areas. In melt curve analysis, a short DNA sequence that matches the bacterial sequence in question is labeled with a fluorescent dye. The labeled sequence, called a probe, emits light only when bound to its target.

The probe and the DNA copies produced by PCR are dissolved together and the solution is slowly heated and cooled. This lets the probe bind to its target. Then the solution is slowly heated until the probe is melted from its target. A special instrument measures the temperature at which the probe melts from the DNA by identifying the temperature at which the probe ceases to emit light. The probe melts at a lower temperature if the target contains mutations and at a higher temperature if there are no mutations in that region of the gene. As a result, the lower melt temperature indicates potentia
'"/>

Contact: David March
dmarch1@jhmi.edu
410-955-1534
Johns Hopkins Medical Institutions
1-Nov-2004


Page: 1 2

Related medicine news :

1. New laparoscopic hysterectomy offers quicker recovery time than traditional surgery
2. Broken heart syndrome: Real, potentially deadly but recovery quick
3. Stressed mice quicker to get skin cancer
4. New female sterilization technique not only quicker and easier for patients, but cheaper
5. Drug-eluting stents adopted quickly, with early disparities
6. Pitt researchers identify biomarkers of lupus which could lead to quicker and better diagnosis
7. HIV infection progresses to AIDS quicker in developing countries
8. Atherosclerosis more common, progresses quicker in HIV patients
9. Corkscrew device retrieves clots, quickly reverses stroke damage
10. Sandia-developed foam likely would stop SARS virus quickly, Sandia/Kansas State team shows
11. SARS outbreak in Beijing spread rapidly, was controlled fairly quickly

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:11/14/2019)... ... November 14, 2019 , ... EMulate Therapeutics, Inc., a clinical-stage ... it will have 5 poster presentations related to its medical devices for treating ... SNO Meeting in Phoenix, beginning on November 20 . , Two ...
(Date:11/14/2019)... , ... November 14, 2019 , ... ... Drug Administration (FDA) has granted acceptance to file status of its application for ... manifestation in the disease neurofibromatosis type one (NF1). The FDA’s acceptance of the ...
(Date:11/12/2019)... , ... November 12, 2019 , ... AI for preventable ... KLAS report “Healthcare AI 2019 - Actualizing the Potential of AI”, which provides ... leader and the only healthcare-focused AI company with enough clients to complete a full ...
(Date:11/12/2019)... ... 12, 2019 , ... The quality of study start-up can ... extended contract negotiations and 80 percent of organizations still using spreadsheets to track ... for improvement. Following an overview of the challenges facing trial sponsors, this webinar ...
(Date:11/11/2019)... ... November 11, 2019 , ... Early praise for A Nurse’s Step-by-Step ... complexities of scholarly writing and provides key insight and vital guidance for publishing dissertations ... will have a permanent place on my bookshelf and will be shared with new ...
Breaking Medicine News(10 mins):
(Date:11/19/2019)... ... November 19, 2019 , ... Continuing the tradition of ... annual inter-hospice countywide breakfast on Tuesday, November 12. The event, which was held at ... branches of the military: Army, Navy, Air Force, Marines and the Coast Guard, as ...
(Date:11/19/2019)... ... November 19, 2019 , ... Dr. David Stoker recently welcomed Carla Crespo, MSHS, ... expertise in nonsurgical and body contouring procedures is recognized nationally, as she is a ... is among an exclusive group of experts in the U.S. who train injectors for ...
(Date:11/19/2019)... ... November 18, 2019 , ... “Impact of the Biofield ... Elizabeth Patric has been named No. 1 International Best Seller and Hot New ... Reference, Research and Theory, and Mind-Body Connection in the USA, Canada, United Kingdom, ...
Breaking Medicine Technology:
Cached News: