"The advantage of using two-photon laser-scanning microscopy is that the short pulsed near-infrared light used to excite the C625 avoids injury to the cell, thus allowing repeated examination of living cells," explained E.J. Bergey, Ph.D., research assistant professor in the UB Department of Chemistry, and a member of Prasad's team.
Generation of the image is done by optical sectioning of cells treated with AN-152-C625, where a beam of light from the microscope is focused on different planes of the cell, one at a time.
"When the AN-152-C625 is outside of the cell, we do not see it because the concentration is too low," said Prasad. "But you begin to see it accumulate on the cell, then in the cytoplasm and finally in the nucleus, with the whole process taking about half an hour."
Other co-authors are Xiopeng Wang, doctoral candidate; Mohammed Al-Nuri, Ph.D., postdoctoral researcher, and Haridas E. Pudavar, Ph.D., postdoctoral researcher, all in the UB Department of Chemistry; Linda J. Krebs, D.D.S., a doctoral student in the departments of Physiology and Biophysics in the UB School of Medicine and Biomedical Sciences and the Department of Oral Biology in the UB School of Dental Medicine, and Saswati Ghosal, Ph.D., of Laser Photonics Technologies, Inc.