CAMBRIDGE, MA--August 8, 2006 Researchers from Phylonix Pharmaceuticals, Inc., today announced results that demonstrates zebrafish is an efficient and effective animal model for assessing human cancer cells at various stages of tumorigenesis. Results were published online today in the advanced online issue of the journal, Angiogenesis. Melanoma, colorectal and pancreatic cell lines proliferated, migrated, formed masses and stimulated angiogenesis in zebrafish. Cells were injected into various sites in zebrafish, including the yolk sac, the brain, and the circulation.
Significant findings of this research include: human cancer cells were not rejected by zebrafish embryos, a major problem with other animal models; new zebrafish angiogenic vessels formed in and around human cancer cell masses, similar to the process of cancer progression in humans; and zebrafish cells incorporated into human cell masses, indicating that cell signaling mechanisms are highly conserved.
The research demonstrates numerous advantages of using zebrafish as an alternative model for cancer research, such as: zebrafish are small, inexpensive to maintain, easily bred in large numbers, require as few as 50 cells for transplant, permit in vivo visualization of cell migration, mass formation and interaction between human cancer and zebrafish cells in the transparent animal, and require small amount of drug per experiment, M vs mM.
Since chemicals can be delivered directly in the fish water and proteins can be injected, assessment of cytotoxic, apoptotic or anti-angiogenic effects of potential drug candidates, singly or in combination, is straightforward.
"Our research demonstrates that zebrafish provide important advantages as a research model, which will be beneficial for advancing cancer research and drug screening,"
said Patricia McGrath, Phylonix' President and Chief Executive Officer. "Although the mouse has been the model of choice for human can
Contact: James G. McManus