Tag: "retroviral" at biology news

Scientists Identify Retrovirus-Like Components In Corn Genome

... their genomes, such as humans, are susceptible to retroviral diseases. Active retroviruses have never been seen...retrovirus-like DNA, corn is not known to have any retroviral diseases," Bennetzen says. "If these retrovirus agents are there, but don't succeed in making the pl...

3-D Structure Of HIV Components May Pave The Way For New Anti-AIDS Drugs

...ugdevelopment for some types of leukemia and other retroviral diseases. Other contributors to the work are Roberto N. DeGuzman, Zeng Rong Wu, andChelsea C. Stalling of UMBC and Lucia Pappalardo of Syracuse University....

Lower Temperature Increases Virus Production

Genetic therapy requires retroviral vectors, which insert the "healthy" genes into the...developed a method of increasing production of the retroviral vectors by a factor of five or 10. This is done by reducing the temperature to 32oC. The new method ...

Researchers Document Transmission Of Protease-Resistant HIV

... South San Francisco, Calif. The technique uses a retroviral vectorthat contains a viral gene segment from the patient's virus and an indicator, tomeasure the sensitivity of the patient's virus to HIV anti-retrovirals. Virologic's phenotypic analysis showed the patient's virus was resistant to tworeverse trans...

Inter-Element Recombination Between Retrotransposons May Be Strategy For Evolution Of Viruses Like HIV

...ould serve as a model system for understanding why retroviral elements evolveso quickly. "This is the f...cDonald. Little is known so far about how retroviral elements establishthemselves and evolve withing the host genome. Analysis of the molecularvariation ...

Hutch Researcher Wins 'Nobel Prize Of Applied Research'

... yielding long-term therapeutic results. While the retroviral vector in the kit is not new, it was used in 1990 ...ional Institutes of Health, it is the first time a retroviral gene expression kit has been madecommercially available. "It's technology that's been around for a ...

Researchers Identify Gene That Sets Boundaries For Heart Chamber Development

...genes wereknown to regulate VMHC1 or AMHC1. Using retroviral vectors to deliver the Irx4gene to the hearts of chick embryos, the scientists asked whether they couldchange how VMHC1 or AMHC1 behaved. They found that Irx4 not only turns on VMHC1,but that it blocks expression of AMHC1. This suggested to Cepko and...

Making A Hand Or A Foot? Tbx-4 And Tbx-5 Are Involved In Determination Of Fore- And Hindlimb Identity

...ositioned at the desired targetregion, the applied retroviral construct carrying the gene of interest,penetrates through the cell membranes forced by the electric field. As a resulta gene(s) can be quickly expressed at a place, where no expression is foundduring normal embryonic development. After several days ...

Fred Hutchinson Cancer Research Center researcher one of five awarded $1 million distinguished young scholars grant

...med at understandingthe molecular events following retroviral infections that lead to cancer.He earned a Ph.D. in medical sciences in 1988 and much to his mother'ssatisfaction a MD degree a year later. He served his residency at Brigham andWomen's Hospital in Boston before starting an oncology fellowship at the...

Research puts sting into drama of virus-cell fusion

...and human T-cell leukemia viruses and is the major retroviral agent used forgene therapy procedures. Funding for the study was provided by the National Institutes of Health,Purdue University Research Foundation and the Leukemia Research Foundation....

New University of Georgia study indicates possible ancient origin for retroviruses, the class to which HIV belongs

...many structuralsimilarities between Cer7 and other retroviral proteins." In fact, Cer7 hasfeatures similar to complex vertebrate retroviruses such as the human T-cellleukemia/bovine leukemia group and the lentiviruses such as HIV. So what? The finding is significant because the retroviral-like Cer7 isin...

University of Pittsburgh discovery of HIV 'shedding' patterns and viral site will change direction of research on AIDS vaccines and treatments

...mportant implications for research on vaccines and retroviral therapies, was presented on Sunday, Jan. 30, in a ...ansmission of HIV in all men. In addition, potent retroviral therapy may have a different effect on HIV in semen as compared to that in blood in men who shed the...

UCSF study reveals mechanism of telomerase enzyme that could lead to target for cancer therapy, cell regeneration

...RNA component of HIV, traditionally disregarded in retroviral therapies as passive, plays an integral role in HIV replication, and it therefore should be closely re-examined as a possible target for therapy, says Blackburn. "This discovery should turn researchers' spotlights back to the RNA components of both...

Different levels of transcription factor coax immune cell progenitors down different developmental pathways

...into PU.1 deficient blood cell progenitors using a retroviral vector. To their surprise, they found that cells that expressed high levels of the transcription factor became exclusively macrophages, while cells expressing low levels of PU.1 developed into B cells. Furthermore, high levels of PU.1 were shown to...

Scientists issue telomerase caution

...e scientists used a genetic trick to eliminate the retroviral copy of the telomerase gene, thus preventing any further telomerase gene expression. Or so they thought. To their surprise, telomerase activity remained high in the cells for at least 20 population doublings after the retrovirus-borne telomerase ge...

Study: Infected people need not carry AIDS virus long before passing it on

...r several days and are sometimes called the "acute retroviral syndrome." The research project involved studying... early as seven days before the onset of the acute retroviral syndrome," Pilcher said. "Each case of acute HIV infection may thus present a unique public health o...

Scientists report first transgenic animal developed via retroviral DNA insertion into male germ-line stem cells

...le germ-line stem cells could be transduced with a retroviral vector and whether any gene introduced would be silenced," Brinster said. "Our work clearly demonstrates that the stem cell can be transduced at relatively high efficiency and expression is not silenced. About 10 percent of stem cells carry active ...

New resources to assist with gene transfer clinical studies

...ters. The Indiana NGVL will produce lentiviral and retroviral vectors; maintain a generally accessible master file that contains toxicology data on adeno-associated virus (AAV), a commonly used vector; and establish a repository to distribute an AAV reference standard to investigators; City of Hope National ...

Gene therapy corrects sickle cell disease in mice, Science authors report

...s researchers had shown to be effective in turning retroviral carriers into better delivery systems to resting stem cells like the blood-forming stem cells in bone marrow. They also optimized the elements surrounding the new gene that control its expression in the red blood cell lineage. Loaded with its cargo o...

Is this the cell that could revolutionise medicine?

...d brain cells. Crucially, using a technique called retroviral marking, Verfaillie has shown that the descendants of a single cell can turn into all these different cell types-a key experiment in proving that MAPCs are truly versatile. Also, Verfaillie's group has done the tests that are perhaps the gold stand...

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