Pinpoint Gene Control Holds Promise For Nervous System Studies
...200,000 replication-defective herpes ampliconviral vector particles into their brains, mice are more activethan their normal counterparts, running around their cages moreand rearing up on their hind legs many times as often as theirnormal counterparts. The genetic alteration also leads to a greater tha...New Nonviral Gene Therapy System Could Help Fight Cancer
...ote anti-canceractivity," Wagner said. The T7 vector gene therapy system is unique because it is anonviral system that works in the cytoplasm of a cell, Wagner said.Other gene therapy techniques work in the nucleus, making themless effective in gene delivery. Another difference between T7 and cur...Trojan Horse Virus Controls Hiv Infection
...hurdle that may allow direct, invivo delivery of a vector that can find its destined target in the body. Thereshould be no need for ex-vivo manipulation of cells." She envisions manypotential applications of this research. Surface-modified live vectors like VSV could be used to shuttle into the bodyviruse...Pitt Researchers Construct Novel Delivery System For Gene Therapy Of Liver Disorders
...e. "We have developed the first non-viral vector that resembles a naturallyoccurring lipoprotein," stated Leaf Huang, Ph.D., professor of pharmacology atthe University of Pittsburgh. Typically, lipoproteins pick up fat and take itto the liver where it is released and processed. "I...New Company To Turn UNC-CH, Army Inventions Into Improved Vaccines
...sease. They also turned the attenuated virusinto a vector -- a delivery vehicle that can be used for many kinds of vaccines.Unlike most vectors, however, this one can be used in the same patient severaltimes. "Many vectors don't work well if reused because the immune system reactsto them," said Davi...Nitrate Reductase: A Magical Enzyme?
...entists to clone a genefrom a plant, put it into a vector that "allows us to manipulate the cloned geneand place it into a yeast where a promoter is 'triggered' by methanol and allowsus to control when the gene is expressed. The end result is an enzyme that isvery much like the natural nitrate reductase we ...Dog Blindness Gene May Help Humans
... the normal gene, inserting a copy into a transfer vector such as a harmless virus and injecting thegene-carrying vector into the back of the eye. The transfer of the normal gene to diseased retinal cells might prevent or...UPMC Researchers Report On Gene Therapy Advances
...d. In their study, researchers employed a viral vector to insert the genes into ananimal's cells. The cells then produced these healing substances both locallyand throughout the body. The researchers found that a single injection of cellscontaining the IL-1Ra gene led to decreased bone loss in the anim......radministration of an adeno-associated virus (AAV) vector containing the gene forcoagulation factor IX. Kat... theintramuscular administration of a gene therapy vector resulting in sustainedimprovement of the clotting abnormality in hemophilia and is an important step...Naked DNA Rejuvenates Rheumatoid Rodents
... usuallyaccomplished by packaging DNA into a viral vector that infects the host cells,carrying with it the desired gene. Such gene transfers are usually transient,and repeated attempts are short-circuited when the immune system recognizes anddestroys the invading virus. Vector-induced inflammation of host ......UCSF investigators created a recombinantpoliovirus vector that provided the first evidence that the reengine...ystem, the researchers reengineered the poliovirus vector toexpress a chicken antigen called Ova. They then inoculated the transgenic micewith this viral vec...Hutch Researcher Wins 'Nobel Prize Of Applied Research'
...ong-term therapeutic results. While the retroviral vector in the kit is not new, it was used in 1990 duringt...line that is used in conjunction with a retroviral vector to create particles that caninvade target cells and deliver the gene of interest. "Clontech was part...Researchers Make Breakthrough In Gene Transfer
...s ofour gene therapy enterprise." This new type of vector might eventually be usedto deliver DNA vaccines, h...n University research groups engineered into agene vector in 1989. To obtain vectors that will not kill cells, the researchers firstinserted an extra ...Viral Vector Delivers Dystophin Gene To Mouse Muscle
...ir immune systems toattack the foreign virus. The vector is called a "gutted" virus, because it hasbeen str...id. "Removing a reporter genecalled LacZ from the vector was the key to reducing the vector's strongimmunogenic effect. Although we still see a small immune...Genetic Code Of Chromosome Of Malaria Parasite Deciphered
...research in malaria pathogenesis,parasite biology, vector biology, diagnostics, immunology, and drug discoveryand development. This research is conducted on the NIH campus and elsewhere inthe United States, Mali, Cameroon, Ghana, Malawi, Thailand, Papua New Guinea,Indonesia and Brazil. **Participation in t...Researchers Announce Breakthrough In Transgenic Animal Technology
...tage. The experimental introduction of genes by a vector - which is structurally verysimilar to a transposable element - into the exposed genome of the oocyteresults in uptake of a new, fully heritable, genetic characteristic. The vectorsused build on the work of the late Howard Temin, a UW-Madison researc...Researchers Externally Regulate Gene Implanted In Brain
...f a gene delivered by thevirus. The viral vector was then injected directly into the brains oflaboratory rats. The protein expressed by the reporter gene served as abiochemical marker that the gene had been transferred successfully. Subsequenttests on brain tissue during three weeks of doxycycline ...UI Researchers Find Potential Gene Therapy For Cystic Fibrosis
...mucous produced by the diseased lungs may trap the vector andprevent it from depositing the CFTR gene in the cells. With chemical assistance,the vector successfully inserts the gene into the cells and CFTR is produced.While the results are beneficial, ...Gene Therapy Incorporates Molecular Rheostat For Controlled, Long-Term Drug Delivery
...ently succeeded in optimizing a gene therapy viral vector toachieve long-term protein production without sparking an immune-system reaction.The vector is an engineered version of a virus known as adeno-associated virus,or AAV, which is a very small, i...Successful Hemophilia B Gene Therapy In Dogs May Justify Human Experiments
...flu-like virus that had been rendered harmlessas a vector to carry the corrected genes into muscle or liver cells where theycould begin producing factor IX, he said. Hemophilia B affects up to about 5,000 people in the United States andpossibly 10 times that many worldwide, Nichols said. Currently, ...