UMass microbiologist's Nature article contributes to understanding of how cells divide
...ecombination that we never knew before." Using E. coli as a model, the researchers looked at mutations in this simple organism with a single chromosome to see how these mutations affected the ability to restart replication. They knew that special sets of proteins called primosome assembly proteins were...Fruit fly's genome sequence finished in record time: Physical maps are the key to accuracy
...e actualstretches of DNA replicated in Escherichia coli bacteria andknown as "bacterial artificial chromosomes" (BACs). Each BACaccurately represents a discrete stretch of the genome, and themap marks each BAC with at least one unique "sequence-taggedsite" (STS) -- ideally with two or more such sites. Usi...Common spices protect bacteria during irradiation
...der, pepper and turmeric prevent destruction of E. coli in tests Researchers in India say that some co...rmeric -- can actually prevent bacteria such as E. coli from being destroyed by irradiation. On the plus side, however, the researchers believe their findin...Study reveals enzyme that behaves like a 'quantum inchworm'
...epers. RecBC is an enzyme that works inside the E. coli bacterium.This nanomachine, one one-billionth of a meter in size, is amolecular motor. It moves along DNA, separating the two sidesof the ladder-shaped DNA so that its rungs can be repaired,their code can be read or they can be paired with another DN...Potato extract: A new direction for antibiotics
...h surface. It also prevented the attachment of E. coli which cause urinary tract infections to its host cells," says Cowan. The researchers have identified the specific compound within the extract responsible for inhibiting bacterial adhesion. It is called polyphenol oxidase, or PPO, and is a common en......, caused by mutations in the adenomatous polyposis coli (APC) gene. The researchers showed that exisulind and other SAANDs induce apoptosis in colon tumor cells by inhibiting the different forms of cGMP PDE in these cell lines. This results in a sustained increase in cGMP in the cells and the inducti...Molecular structure suggests how a gene can "jump"
...es the 3-dimensional structure of the Escherischia coli Tn5 transposase bound to the Tn5 transposable element. "Our discovery is an important step in understanding the structural basis for transposition," says Rayment, a crystallographer and molecular biologist. The team's findings have implications for ...Understanding how E. coli kills cells
...se scientists have uncovered how the pathogenic E. coli bacteria cause cell death in their human hosts. Th...hibiting cell death caused by the bacteria. The E. coli bacteria include pathogenic strains that are major causes of diarrhea in developing countries, killi...Genetic 'switch' key to biotech patent royalties
...e switch in the bacteria E. coli. The strain of E. coli used in Keller's research differs from the beneficial strain existing in the digestive tract of humans or toxic strains that can exist in poorly prepared food. Now researchers at Life Technologies Inc., a Maryland laboratory under contract with Res...'Gatekeeper' protein is key to cellular life; links bacteria, chloroplasts and mitochondria
...re investigating the protein's role in Escherichia coli bacteria as part of a National Science Foundation-... Dalbey and Samuelson developed a strain of the E. coli in which YidC can be depleted. They found that when the YidC was absent, proteins could not migrate ...New treatment makes fruit juices safer
... orange juice and apple cider with salmonella, E. coli and lysteria monocytogenes, another bacteria commonly found in food, and then treated the contaminated juice with the pressurized CO2. The treatment reduced the bacteria population from more than 100,000 organisms per milliliter to zero, according to......crystallized a glycerol-conducting channel from E. coli and analyzed the structure in atomic-level detail using x-ray crystallography.The images of the crystal structure actually caught the glycerol molecule in the process of passing through the membrane's protein channel. This "midstream" capture had nev...American Society for Microbiology news tips for November 2000
...rnal of Clinical Microbiology, 38: 4274-4276.) E. coli bacteria find their way inside apples The virulen...nd the apples placed in plastic bags containing E. coli O157:H7 bacteria. They were then tested for bacterial contamination at the puncture wounds, on inta...Tipsheet: Journals of the American Society for Microbiology
... the virus uses for replication with genes from E. coli bacteria, widely used to regulate gene expression in other applications. The result is an attenuated HIV that requires the antibiotic doxycycline in order to replicate, and the concentration of the antibiotic regulates the rate of replication. "It i...Genome project finds the 'triggers' for E. coli illness
...ison -- The newly completed genomic sequence of E. coli O157:H7 reveals how these potentially deadly bact...ntrol the public health threat. This strain of E. coli O157:H7 causes an estimated 75,000 illnesses a year in the United States, including numerous deaths,...Plants as plants: gene could convert crops to plastics factories
...project near completion that uses the bacterium E. coli to produce the monomer for a type of plastic that is used in carpet fibers, among other things. However, plants are more attractive chemical factories than microbes because they may be cheaper. The inherent advantage with plants is that you get your ...Computer model predicts outcome of DNA shuffling
...chers then place recombined genes into Escherichia coli to identify which new genes produce usable or potentially interesting products. Those genes that express a potentially interesting protein or enzyme are again fragmented and reassembled to form new recombinant genes. The process continues until a p...Tipsheet: April journals of the American Society for Microbiology
...Asfaw. 2001. Identification of fecal Escherichia coli from humans and animals by ribotyping. Applied and Environmental Microbiology, 67: 1503-1507.) Influenza treatment may enable resistance The influenza virus may be developing resistance to a drug recently approved for the prevention and treatm......icle, "Enlarging the Amino Acid Set of Escherichia coli by Infiltrating the Valine Coding Pathway," is aut...a general method to make the bacterium Escherichia coli incorporate novel amino acids site-specifically. Their approach starts with generating an orthogonal......mistry and biophysics at Texas A&M, studies how E. coli bacteria move to find food. E coli is attracted to nutrients such as the sugar maltose and repelled by harmful substances like nickel i...