Anthrax enzyme images reveal secrets of antibiotic resistance, suggest new drug design
...PS using X-ray crystallography. The team bombarded crystals of the enzyme with X-rays, then used the patterns formed by the diffraction of the beams off the crystals to create computer-generated, three-dimensional images of the enzyme shape. The researchers also use...Images of 'tail' of protein needed for cell multiplication suggest anticancer drug targets
...rns formed by the diffraction of the beams off the crystals to create computer-generated, three-dimensional images of the shape of the bonded proteins. However, images of the structure created using X-ray crystallography failed to provide detailed views of half of the individual amino acid building blocks ma...How an insidious mutation fools DNA replication
... protein and the DNA in the crystal. The series of crystals they analyzed constituted snapshots of the polymer...tered a surprise when they analyzed the polymerase crystals with either the correct or mismatched nucleotides. "We saw that, ironically, when the polymerase bin...Scientists visualise cellular handmaiden that restores shape to proteins
...hotosystem II, published last year in Science. The crystals of chaperonin complex were grown and prepared in Iwata's lab, and after X-ray analysis at the European synchrotron facility, all authors collaboratively solved the structure....UC Riverside researcher takes snapshots of the movement of molecules in a billionth of a second
...y role in processes as diverse as the formation of crystals and the activity of catalysts. "This is very basic research but it has implications for many other areas in science," said Bartels. "Catalysts, like the one in the exhaust system in every car, are made from a porous material. The exhaust gas is pas...Newly designed nanoparticle quantum dots simultaneously target and image prostate tumors in mice
...dots are nanometer-sized luminescent semiconductor crystals that have unique chemical and physical properties due to their size and their highly compact structure. Quantum dots can be chemically linked (conjugated) to molecules such as antibodies, peptides, proteins or DNA and engineered to detect other mole...New milestones on the path to conquering HIV drug resistance (AIDS)
... by which X-ray diffraction patterns obtained from crystals are used to map the three-dimensional atomic structure of large molecules. "We have been fighting this battle together for more than 15 years," said Hughes. "We are both very excited about developing a better understanding of the fundamental biolog...Molecular image of genotoxin reveals how bacteria damage human DNA
...tion. It took Nesic nearly two years to obtain the crystals she needed for X-ray crystallography - a technique that records the pattern produced when X-rays bounce off a target protein crystal. "You have to find the right conditions and the right concentrations for crystallization," said Nesic. "You start wit...Evidence of nanobacterial-like structures found in human calcified arteries and cardiac valves
...use compared to controls containing hydroxyapetite crystals they "incorporated radiolabeled uridine in a time-dependent manner of three days, providing evidence of ongoing nucleic acid synthesis." One interpretation of these results, especially given such potential parallels as H. pylori causing ulcers, could......he particles, the team says. However, even apatite crystals alone seemed to absorb some uridine, though not as much as the self-replicating particles. And when the Mayo team doused their tissue samples with an antibody that Nanobac claims binds to a protein unique to nanobacteria, they found it bound to disea...Protein keeps cold fish from becoming frozen flounder, new study shows
...eins (AFPs) do this by binding irreversibly to ice crystals and preventing them from growing. Until now, it h...xcluded," explains Mr. Marshall. "Lemon-shaped ice crystals that differed significantly from the hexagon-shaped crystals obtained with type I AFPs told us that ...Ferr-D'amar wins 2004 Eli Lilly and Company Research Award
... First, he developed new methods to make obtaining crystals of RNA much easier. RNA crystallization is a very ...Ferr-D'Amar developed new techniques to make these crystals form much more readily than by earlier methods. He then used the technique of X-ray crystallography ...Breakthrough Clemson research appears in Science
...the research team concluded that the intracellular crystals are used to form shell. They observed rhombohedral-shaped crystals, leading the researchers to theorize that they were calcium carbonate -- a basic substance in hard tissue. They also observed the number of crystal-bearing cells increased 300 percent...'Crystal engineering' helps scientists solve 3-D protein structures
A new technique for engineering protein crystals is helping scientists figure out the three-dimensional structures of some important biological molecules, including a key plague protein whose structure has eluded researchers until now. The technique, developed with support from the National Institu...How DNA copying enzyme 'stops the presses' for repair synthesizing enzyme
...s. Importantly, said Beese, the loosely associated crystals were so constructed that the polymerase could actu...rase to be error-prone, so that they could produce crystals with mispaired bases inserted in the active site. What's more, they were able to move the mismatch ......h Quantum Dots. These are nano-sized semiconductor crystals a mere ten millionth of a millimeter in diameter t... colors upon excitation with a laser source. These crystals enabled the researchers to deliver real-time video-clips of signal transmission in the so-called erb...Microbe's trick provides a template for willowy crystals
...her novelty: microbes that produce nanometer-scale crystals of extraordinary length. The discovery of the willowy microscopic crystals may open a broad new window to human understanding of biomineralization, the same process that produ...Capturing cell protein production in action could help fight antibiotic resistance
...he difficult and time-consuming process of growing crystals that can be analysed using X-ray diffraction. Electron microscopy images are created by firing electrons at the sample but this process rapidly damages the biological material. To overcome this degradation problem researchers use a low dose of radia......is separated from DNA." To find good diffracting crystals out of the hundreds made, the researchers used a n... automated screening system stores the tiny frozen crystals on nylon loops at the end of metal pins. A robotic arm retrieves each pin and aligns the crystal in ...