"A Thousand (And More) Points Of Light" Yield New View Into Cells
...havelinked up (hybridized) with their RNA partners fluoresce and are detected usinglight microscopy. Digital images of the fluorescent points are acquired through"optical sectioning" of the hybridized cell. The thousands of points of light(photons) acquired in this way go in and out of focus. The out-of-focus ...How Can You Tell Whether Corn Is Fungus-Ridden?
...ain species offungus glow in the dark. Grains that fluoresce bright green-yellow are thrownaway. The trouble is that this BGYF test, as it's known, misses around 15 percent of infected kernels. So chemists Sherald Gordon and Richard Greene of the National Center forAgricultural Utilization Research in...Quick, easy, color-coded technique will speed identification of drugs for diseases
...nance energy transfer) would occur and they would fluoresce yellow. If the receptors were activated, these tags would drift apart and they would show up as cyan under the microscope. Using a one-celled amoeba called Dictyostelium, whose GPCRs serve as docking bays for a chemical known as cyclic AMP (cAMP), ...... the coenzyme that causes methanogenic bacteria to fluoresce blue-green. White has shown that F420 formation uses four previously unknown enzymes, one of which uses a reaction like that found in DNA polymerase, thus establishing a link between the genes and the coenzymes. "We can extrapolate from this informat...Worm neuron research may lead to powerful model for Parkinson's study
...ine neurons, which had been genetically altered to fluoresce green, had been destroyed by exposure to a chemical, 6-hydroxydopamine (6-OHDA). The results of the study, published this week in the journal Proceedings of the National Academy of Sciences, suggest that this tiny roundworm, named C.elegans, can serv......reates what we call the coral reef. Living corals fluoresce in several colors when illuminated with blue light, and the ways these fluorescent colors mix produces a wide range of coral appearances. But here's the rubscientists still aren't sure why the animals do it. "This is not bioluminescence," says Ackl...Carbon nanotubes found to fluoresce
...uld bind only to the target cells. Since nanotubes fluoresce with a single wavelength of light, and different diameter nanotubes give off different wavelengths, it may be possible to tailor different sizes of tubes to seek specific targets, and thus diagnose multiple maladies in a single test using a cocktail ...'Glowing' technique could detect river pollution
...ing energised. For example, glow in the dark toys fluoresce after being energised by ultra-violet daylight. River waters fluoresce at wavelengths that the eye cannot see. They would also seem to glow in the dark if our eyes were s...Genetically engineered mice offer hope for isolating beta cells and treatment of type 2 diabetes
... they can be detected in living cells because they fluoresce brightly upon exposure to ultraviolet light or blue light without the addition of coactivators or external substances. In addition, pure populations of fluorescent-tagged cells can be isolated using a fluorescence-activated cell sorter. Rat and human...Engineered proteins will lead to 'synthetic biology'
...rophore, attached such that the protein would only fluoresce if the target molecule had plugged into the protein. The resulting sensor proteins proved in test tube studies to have high affinity and specificity for their target molecules, said Hellinga. For example, he said, both the TNT-sensing and serotonin-s...'Kiss-and-run' rules the inner lives of neurons
... protein, developed by other researchers, does not fluoresce under acidic conditions normally present in vesicles fully loaded with neurotransmitter. However, when the vesicle releases its payload, the interior becomes less acidic and the vesicle glows a bright green. Thus, said Stevens, by imaging individual ...Protein is shown to aid cell's reading of genetic code
...odies that attach to Pol II and FACT molecules and fluoresce in different colors under ultraviolet light. With an optical microscope, the researchers could then see the location of Pol II and FACT on the DNA strand. By introducing the cross-linking at various times, they created a step-by-step record of the p...Mantis shrimp fluoresce to enhance signaling in the dim ocean depths
...down, so on the ocean floor the crustacean's spots fluoresce yellow-green to enhance their prominence in the dim blue light. Though fluorescence in marine animals is common - in corals and squid, for example - this is the first documented case of fluorescence used in signaling in the sea. Fluorescence occurs w...JCI Table of Contents, January 15 2004
..., cells derived from bone marrowderived stem cells fluoresce green, while those cells that reside in the lung do not. Most of the collagen-producing fibroblasts observed in the lungs of these mice fluoresced green, indicating that they were of bone marrow origin. In an accompanying commentary Sarah Dunsmore an......ere ten millionth of a millimeter in diameter that fluoresce in several different colors upon excitation with a laser source. These crystals enabled the researchers to deliver real-time video-clips of signal transmission in the so-called erbB receptor family, important targets for many anti-tumor drugs such as...Fundamental change to Immunology 101
...y-one past a bank of lasers, detecting which cells fluoresce in a certain way based on a prescribed molecular tag. The suspected early progenitors (HSCs, MPPs, and CLPs) had known differences in cytokine receptors, so the team used these as molecular tags to characterize the different cells present. ...Revealing bizarre deep-sea secrets
...portant because the proteins that allow animals to fluoresce are used in genetic research and new fluorescent animals may contain proteins that offer novel benefits in such work. Dr. Mazel will use powerful lights mounted on the front of the submersible to illuminate animals whose fluorescence will then be cap...Gold quantum dots have applications in biological labeling, nanoscale optics
... how they share conduction electrons, and how they fluoresce under quantum confinement. "They can help us understand the very small size scale that is really not well understood for noble metals," Dickson added. "They can provide the 'missing link' between atomic and nanoparticle behavior in these metals."...Chemical derived from vitamin-E shows early promise as cancer drug
...y of Texas Health Science Center in San Antonio to fluoresce bright green under proper exposure. Using this fluorescent cue, Kline and colleagues determined that mice treated with aerosolized alpha-TEA had less than half the number of lung micrometastases as untreated mice (an average of 31 versus 73 de...