Research suggests new avenue for stopping, preventing colon cancer
...est it in mice to see if it has the same effect as knocking out the gene. "We've been carefully examining drugs which inhibit COX-2," DuBois explained. "Now we can really focus on key components of the downstream pathway." The research could lead to more specific and safer -- ways to prevent colorectal cance...Essential smell gene may provide key to new insect repellents
...eneral one, underlying the fly's ability to smell, knocking it out would blind the insects to all odors. Mutant flies lack a sense of smell The researchers used a technique called gene targeting to create a strain of fruit flies lacking the Or83b gene. The flies appeared to be normal, but they could not sm...Rutgers-Newark biologist links presence of protein to spread of cancerous cells
...c effect on this particular Rho pathway instead of knocking out all cells that are dividing."...Transcriptional gene silencing in nucleus shown by UCSD/VA medical researchers
...tial to modify gene expression in disease, such as knocking out expression of genes required for tumor growth. He cautioned, however, that further studies are needed to prove the general applicability of this concept. An understanding of siRNA begins with a look at theway by which genes work. First, a "pr...Researchers discover how worms' noses sense oxygen
...ed as a prototype for further nematode studies. By knocking out several genes coding for parts of the guanylate cyclase enzyme, they were able to show that this enzyme was acting as an oxygen detector, primarily steering worms away from too much oxygen. The enzyme is found in three separate neurons that inner...'Mighty mouse' gene works the same way in people
...s, too." Lee and his team discovered in 1997 that knocking out the myostatin gene led to mice that were twice as muscular as their normal siblings, lending them the moniker "mighty mice." Later, others showed that naturally bulky cattle, such as Belgian Blues, got their extra muscles from lack of myostatin, ...Newly discovered gene controls levels of 'bad' cholesterol in mice
...produced the first mouse model of heart disease by knocking out, or deleting, the apo E gene. The mouse provided the scientific community with the first small animal model to study heart disease in a controlled fashion that can render discoveries relevant for the human condition....Rb protein's role in retina development is key to understanding devastating eye cancer
...ne function. These techniques included methods for knocking out Rb from retinal cells that can be studied in a laboratory dish, as well as methods for knocking out Rb in single retinal progenitor cells so the effect of this mutation could be studied in both em...Jefferson researchers uncover biochemical clues to how cells migrate in embryos
...tinued along the pathway, step by step, chemically knocking out enzymes and replacing them with their products to see if they could restore the normal pathway. They subsequently determined that blocking a particular enzyme, geranylgeranyl transferase I, which is further along the pathway from HMG-CoAReductase...Researchers adapt RNA interference to study gene function on a large scale
... to cultures of Drosophila cells and assayed how knocking down the function of a targeted gene affected cell numbers in the cultures. This basic measure, said Perrimon, revealed genes that are not only involved in general cell growth, but also in the cell cycle, cell survival and other such functions. The r...Gene may protect against heart disease rather than be harmful as previously thought
...the first time in the mouse by the Breslow team by knocking out, or deleting, a gene called Apo E. These mice are known as Apo E knockout mice. The severity of atherosclerotic disease is seen in varying degrees among different strains of Apo E knockout mice. Genetic differences cause them to have different ra...Breast cancer can be reversed in laboratory mice, scientists report
...iate breast tumors. The new work demonstrates that knocking out the beta-1 integrin gene prevents cancer-prone mice from developing breast tumors, and halts further tumor growth in mice that have already developed breast cancer. "This study shows that it is absolutely essential to have the beta-1 integrin gen...Better model of cancer development sheds light on potential angiogenesis target
...del in which you study a process," says Alani. "If knocking out the Id1 gene in two different models produces two different results, then we need to reevaluate the role that Id1 plays in angiogenesis." In the model using skin carcinogen exposure, the team's preliminary findings suggest that cancers may devel...And the beat goes on: New insight into the genetics of congenital heart disease
... the heart has formed. Dr. Jiao hit on the idea of knocking the Bmp4 gene out just in the embryonic heart muscle (cardiomyocytes), leaving it intact everywhere else. What is more, he manipulated the system, using 'conditional tissue specific gene inactivation', so that Bmp4 activity could be titered down to d...Stem-cell defect underlies common genetic disorder
...croarrays for identifying activated genes and then knocking out those activated genes in mice to determine how those genes regulate stem cell function. "We think that this represents a powerful combination for getting important insights into the causes of other types of birth defects or other types of disease...Creation of new neurons critical to antidepressant action in mice
...play an important role in regulating emotions. By knocking out the gene that codes for a key subtype of serotonin receptor (5-HT1A), the researchers created a strain of "knockout" mice that as adults show anxiety-related traits, such as a reluctance to begin eating in a novel environment. While unaffected by...Researchers identify new cause of genomic instability
... and other threats. To explore the implications of knocking out the H2AX gene, lead author Craig Bassing created a line of mice lacking both copies of the H2AX gene. "Both Craig in our lab and Andre Nussenzweig at the National Cancer Institute produced knockout strains that showed an increased level of ge...New approach to gene knockouts reveals the 'master planners' of the skeleton
...lling mammalian development to test the effects of knocking out multiple members of the Hox gene families. K...ellik and Capecchi attempted the difficult task of knocking out all of the Hox10 or Hox11 paralogous gene forms, or alleles. The experiments were particular...Strategy to treat AIDS patients in Africa with less drug, reduced toxicity set for clinical test
...nd principal investigator on the study. "Now we're knocking on other doors to try to keep this study on track. Most people, including myself, would never have expected such a crucial study to be in peril at this stage of development." The reasoning behind the NIH policy is that the high cost of providing drug......velopment. "We learned how these genes function by knocking out the gene," Murphy said. "This is the genetic equivalent of taking a car from the assembly line and just pulling out a particular part. When the car is finished without the part, you see what works and what doesn't. "In this case, we have removed ...