Tag: "ligation" at biology news

Sweet success in targeting sugar molecules to cells in living animals

... the azido sugar into mice and used the Staudinger ligation to attach a phosphine molecule that carried a dist...raphy. They are also developing new bio-orthogonal ligation reactions with azides that will give them additional sugar-tagging techniques....

When is a mouse like a test tube?

...ate the exterior of cells, then use the Staudinger ligation to attach phosphines to these azides. If a label, ...eared on spleen cells, proving that the Staudinger ligation had occurred in living mice. "We successfully marked cells as a function of the robustness of a meta...

The first engineering of cell surfaces in living animals

...nd her colleagues have demonstrated the Staudinger ligation in remodeled cells of living mice. The ability to ...ave no harmful biological effects. The Staudinger ligation meets these requirements. It is a reaction between a functional group called an azide (a class of co...

JCI table of contents, 1 June 2004

...ring multibacterial peritonitis induction by cecal ligation and puncture. This protection was lost in RAGE/ mi...n Induces Transplantation Tolerance TITLE: CD28 ligation induces transplantation tolerance by IFN-_____dependent depletion of T cells that recognize alloanti...

JCI Table of Contents, September 15, 2003

...ITLE: Biosynthesis of 15-deoxy-?12,14-PGJ2 and the ligation of PPAR-gamma AUTHOR CONTACT: Garret A. FitzGerald Center for Experimental Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania, USA. Phone: (215) 898-1184 Fax: (215) 573-9135 E-mail: garret@spirit.gcrc.upenn.edu View the PDF of thi...

New study clarifies connection of brain and heart failure

...athetic nerves in rats with a large MI produced by ligation of the left anterior descending coronary artery and confirmed by echocardiography. Results The researchers found the characteristic features of heart failure that were present in MI rats with sham AV3V lesion, increased sodium appetite, the decrea...

Applied Biosystems announces SNPlex ultra high throughput genotyping system

...on Applied Biosystems' proprietary oligonucleotide ligation reagent and electrophoretic detection technology. This technology is also employed in the Celera Diagnostics cystic fibrosis test, which has become one of the leading tests for detection of mutations associated with this disease. The SNPlex TM syst...

FDA clears Berlex Laboratories Mirena (R), new form of long-acting contraception meets need for U.S. women

...t is as effective in preventing pregnancy as tubal ligation (better than 99 percent) and lasts for five years, or until removed. Available for 10 years in Europe, MIRENA has been used by approximately two million women worldwide and will be available for the first time in the U.S. in the first quarter of ...

A new way to engineer cells: The Staudinger ligation

...f California at Berkeley, describes the Staudinger ligation in the March 17 issue of Science magazine. Ber...x natural sugars that crowd cell surfaces. Through ligation with specific reagents, the markers provide new ways of labeling cells as targets for cancer therapy...

Everyday Technology Underlies First DNA Computer Logic Gates

...lace biological laboratory techniques, such as DNA ligation and gel electrophoresis. "There is absolutely nothing fancy in what we have done," Ray says. "The techniques we've used are the same ones that thousands of biologists use every day in their labs." Ray, an assistant professor of biology,...

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