Unraveling a protein, researchers uncover mechanics of anti-cancer agent
...ned a mystery. Using crystallography, atomic force microscopy and advanced computer modeling, researchers sorted the chemical structure and mechanical strength of the known fibronectin proteins that glue together myriads of cells in mammalian body tissues. In the Dec. 9 issue of the Proceedings of the National ...Multiphoton microscope observes cell membrane action
...r experiments -- based on 3-D-resolved multiphoton microscopy to illuminate membrane domains and transition beha... a Keck Foundation grant to Baumgart. Multiphoton microscopy is a patented process developed at Cornell by Webb, who also is the Eckert Professor in Engineering ...NSF awards four-year, $3.9 million grant to plant biology team led by University of Georgia
...d for meristem function in maize. Laser dissection microscopy (LM) is a powerful technique that permits the isolation of RNA from specific cell types within fixed plant tissues immobilized on slides. RNA collected from 1,000-10,000 cells is sufficient for use in analyses of gene expression, which permit the sim...Building a better atlas of yeast proteins
...f specificity. "First, we examined the cells using microscopy techniques, and for many proteins that was enough. We could tell that many proteins -- more than a thousand -- existed in the nucleus or the cytoplasm." The nucleus contains the cell's genetic material and the cytoplasm is the fluid-filled region out...Pathways of emotion from cortex to peripheral organs
...es to the spinal cord. The team then used electron microscopy to look at nerve endings in the hypothalamus. They saw that neurons from one area of the prefrontal cortex terminated in large 'boutons' in the hypothalamus. The appearance of these boutons suggested that the prefrontal neurons have an excitatory eff...Purdue team solves structure of West Nile virus
...nding of the virus' life cycle. Using cryoelectron microscopy and advanced imaging techniques, the Purdue team has determined the orientation of the major surface proteins in a West Nile viral particle. Because these proteins are instrumental in allowing the virus to bind to and invade a host cell, the research...Brookhaven Lab develops 'ThraxVac' to clean up anthrax
...struction process using both transmission electron microscopy and scanning electron microscopy at Brookhaven. So far, test results are extremely promising. The final steps before commercializatio...Salmonella uses molecular staples to change structure of infected cells
...and the Virginia researchers developed an electron microscopy image of SipA-actin filaments - an arrangement too...ructure upon the larger and less detailed electron microscopy representation, and through extensive image processing, tease apart the role that SipA plays in form...The American Academy of Microbiology releases report
...at should advance our common progress, such as new microscopy techniques for looking at communities and genomic techniques that allow us to see what genes are being expressed in communities at any one time." The report discusses the importance of microbial community functions to human and environmental concerns...Data privacy, emergency response, weather prediction to benefit from information technology advances
..."family tree" for life on Earth; harness automated microscopy and data mining of biological image databases to observe and understand cellular and molecular processes; and simplify the way scientists develop applications for grid computing. "This year's ITR awards demonstrate how fundamental computer science re...As autumn approaches, this chickadee's brain begins to expand
...adult] brain." Saldanha uses transmission electron microscopy (TEM) to examine neurons (nerve cells) and synapses (connections between nerve cells, where scientists think learning occurs) from the brain of the black-capped chickadee. His goal is to determine whether estrogen is being made in the cellular body o...Atomic force microscope used to measure how well live bacteria stick
...earchers are using a modified form of atomic force microscopy (AFM) to observe at subatomic levels the efficiency of the attachment of bacteria to silica surfaces. The geological scientists are simulating environments similar to ground water in sandy soils. Sticking efficiency of bacteria has not been previousl...Findings in frog oocytes may help study of chromosome physiology
...s, XCAP-E and XCAP-D2, and confocal laser scanning microscopy to zero in on the proteins' precise locations inside the nuclei of gametes of the African frog Xenopus laevis. (The research will be on display as a poster presentation Aug. 17-22 during the Gorden Research Conference on Plasmid & Chromosome Dynamics...Novel bacterium detoxifies chlorinated pollutants
...y techniques and high-resolution scanning electron microscopy yielded information about the organism's appearanc...nt Kun-Lin Yang, who contributed scanning electron microscopy studies, and Stephen Koenigsberg of California-based Regenesis Bioremediation Products, which contri...Rapid movements of living biomolecules visualised
...n of different biomolecules. For this he used FRET microscopy (Fluorescence Resonance Energy Transfer). When two fluorescent molecules approach to within several nanometres of each other, one molecule can transfer energy to the other. This energy transfer causes a change in the colour of the fluorescence. Molec...DNA unwinding protein runs on two motors
... 2001, Kowalczykowski and colleagues used advanced microscopy techniques to film a single molecule of RecBCD unwinding DNA in real time. The ends of a single strand of DNA are called three-prime (3') and five-prime (5') respectively, because of the way the sugar molecules that form the backbone of the DNA are j...Organelle's discovery challenges theory, could alter approach to disease treatment
...id. Using transmission electron and immunoelectron microscopy and X-ray microanalysis on the bacterium, researchers got a highly magnified and illuminated view. They applied a fluorescent dye into the suspected organelle. They saw a membrane around it. The dye stained areas only within it, not in the cytosol....UT Southwestern researchers find immune system stages for eliminating viral infections, cancer cells
...UT Southwestern researchers used three-dimensional microscopy in real-time to visualize the subcellular localization of components of NK cells during the in vitro killing of tumor target cells. By learning that cellular organization has to go through a defined series of steps to kill a target cell, researchers ...'Kiss-and-run' rules the inner lives of neurons
...about how vesicle retrieval occurs. Early electron microscopy images of vesicles in synapses were interpreted as either a kiss-and-run model or one in which the vesicle is completely incorporated into the cell membrane, to be drawn back into the cell. "The advance that we have made is to figure out a way of ima......sor of applied physics, co-inventor of multiphoton microscopy (with Winfried Denk) and leader of the experimenta...signal-to-noise illustrates how useful multiphoton microscopy with quantum dots can become, in a biological research context, for tracking cells and visualizing t...