Molecular staples shape a cancer killer
...ling their cellular uptake, the hydrocarbon staple overcomes classic shortcomings of peptide therapeutics. As the molecular events that lead to execution and death have become clearer during the last decade, researchers speculated that it would not be long before biotechnology and pharmaceutical companies w......A) describe a way to copy mass amounts of DNA that overcomes some of the limitations of this earlier technique. The new technique is called HDA (helicase-dependent amplification). HDA is as simple as PCR, but has significant advantages. PCR requires thermocycling to heat and cool a sample of DNA, to allow den...Carnegie Mellon U. develops microgel to recover enzymes for manufacturing, research assays
...ng efficient enzyme recovery, this microgel system overcomes significant obstacles in using natural enzymes in laboratory and industrial settings," said Bruce Armitage, associate professor of chemistry at Carnegie Mellon, who developed the recoverable enzymatic microgel in collaboration with chemistry professo...Capturing cell protein production in action could help fight antibiotic resistance
...al part of many structural biologists' toolkit. It overcomes the problem of weak image contrast in electron microscopy and avoids the difficult and time-consuming process of growing crystals that can be analysed using X-ray diffraction. Electron microscopy images are created by firing electrons at the sample ...Carnegie Mellon enhances Quantum Dot Corp. technology for long-term, live-animal imaging
...scence in a living animal. This technological feat overcomes a major limitation, making quantum dots finally practical for long-term studies in mammals. Reporting in the January/February issue of Bioconjugate Chemistry , Byron Ballou and colleagues at Carnegie Mellon, in collaboration with the Quantum Dot Co...Newly identified gene may halt fuzzy, moldy fruit
... molecular pathway that determines whether a plant overcomes adversity. "In terms of biotechnology and improvements of both agricultural and horticultural plants through molecular approaches, it's important that this gene is a regulatory protein because it means you can just alter it so there is more or less o...Proteins enable HIV to override cell's defenses
...hese proteins is the key for understanding how HIV overcomes host defenses and could potentially be new targets for HIV medications. A study detailing the finding is published in the October 16, 2003, online version of the journal Science. As the researchers explained in their article, viruses like HIV contain...Penn researchers identify secondary pathway for survival of cancer cells
...cting foreign growths, such as a new organ. Pim-2 overcomes this potent inhibitor of cell survival. While these pathways appeared distinct, they are not completely different and both have some common actions. One factor shared by both pathways is a phosphorylated "off signal" suppressor protein called 4E-B...Technique brings immune-based therapies closer to reality
...logical advance reported by the Johns Hopkins team overcomes a major weakness of current methods for making these targeted immune cells, known as antigen-specific cytotoxic T cells (CTLs) -- namely the methods' reliance on a patient's own dendritic cells. Dendritic cells are immune system sentries that wave th...Virginia Tech inventions, creations can improve lives
...the state of the flow." Using a fiber optic sensor overcomes problems from temperature and electromagnetic fie...rate positive and negative charges), the invention overcomes these limitations. The method provides a thinner film and precise structural control, with at least ...Researchers develop 'natural bandages' that mimic body's healing process
...ly increased until the force of the electric field overcomes the surface tension of the solution. This forms a liquid jet that is transformed into a dry fiber before it reaches the target. The solution is made with a high concentration that causes the polymer chains to intertwine. Instead of breaking into drop...A bed of microneedles: Johns Hopkins scientists' gadget measures muscle cell force
...o measure forces exerted by a contracting cell and overcomes some of their limitations, the researchers say. For example, one common method examines a cell lying on a thin sheet of material, which wrinkles when the cell contracts. "This is like a person lying on a bed sheet and scrunching up part of the shee...Stanford researchers develop gene therapy technique that sharply cuts risks
...nline edition of the journal Nature Biotechnology, overcomes the need for viral vectors that have plagued gene therapy trials, while retaining the ability to insert therapeutic DNA into specific sites in the chromosomes. "Our approach provides an alternative that didn't exist before," said Michele Calo...New robotic microscope helps scientists track cells over time
...F assistant professor of neurology and physiology, overcomes this problem. At first glance, the robotic microscope looks like an ordinary inverted microscope. The differences are in the fast and precise motors and sophisticated computer programs that automatically focus the objective, move the stage, and photo...New research may lead to more effective treatment of asthmatic attacks
...and a counterelectrode. When the electrical stress overcomes the surface tension of the liquid, a cone is formed, from which a thin jet emerges. The jet breaks up into monodisperse droplets. The device spray section in the experiment used a nozzle-ring configuration. The nozzle and the ring were connected to ...After hooking toxin behind seafood poisoning, Science researchers may tackle prevention
...h, according to the study. The Science research overcomes the previous challenges of isolating ciguatoxin from nature. Although very potent, the actual amount of collectable toxin is very low in fish, and cultivating natural samples of the chemical proved too unreliable to facilitate public health research....New lab-made stem cells may be key to transplants
...tential to develop into different tissues but also overcomes great drawbacks that have limited the use of hPSCs for disease therapy. The new cells, called embryoid body derived cells (EBDs), "will be the workhorses that carry out the new tissue-transplant therapies," says John D. Gearhart, Ph.D., the Hopkins...Ion microscopy pinpoints drugs inside cancer cells
... a method called "sandwich fracture." This method overcomes compositional and conductive problems and allows cells to be studied for their intracellular chemical composition. The ion microscope was invented in 1962 and originally was exploited in the semiconductor and electronics industry by such companies as...New fertility technique to help women have own genetic baby from donor egg
...donated egg and transferring the patient's nucleus overcomes potential problems that could arise from accidental mixing of important extranuclear genes (mitochondrial DNA) from patient and donor. "Because ICSI will probably be the best way of fertilising the reconstructed eggs the mechanical method we've de...Protecting the rice bowl: Chromosomal mapping of the rice blast fungus
...ts host rice plants andparticularly how the fungus overcomes host defense mechanisms. This knowledgeis essential not only for combating rice blast but for understandinghost-pathogen interactions in general....