Tag: "retroviral" at biology news
Genetica, Inc. develops high throughput RNAi tool for drug target validation
... particularly applied one of these technologies, a retroviral
tool called CELLx, to antibody production. Gregory Hannon, Associate Professor at Cold Spring Harbor Laboratory, principal investigator in the new study, and a founder of Genetica says, "RNAi has emerged as a highly promising research tool for determ...
Unexpected links found between many RNA viruses
...d in a structure called the plasma membrane, where retroviral
replication takes place. This replication machinery is triggered by an RNA packaging signal that parallels the action of the signal in BMV. These same principles of sequestering a viral RNA intermediate and its polymerase, plus other features of [+]R...
Retroviruses shows that human-specific variety developed when humans, chimps diverged
...ur genes than genes in our genes." Just how these retroviral
elements have moved around in the human genome and...her than simply playing a role in human evolution, retroviral
elements may actually be implicated in the leap from chimpanzees to humans. Until a mere 50 years ag...
Gene therapy treats first disease affecting multiple organ systems in a large animal
...I received four simple intravenous injections of a retroviral
vector expressing canine beta-glucuronidase. The vector used was made from the Moloney murine leukemia virus with a liver-specific promoter; at several days of age, canine liver growth is so rapid that transduction occurs readily and beta-glucuronid...
JCI Table of Contents, December 2, 2002
...ts withautosomal recessive hypercholesterolemia by retroviral
expression ofARH1 CONTACT: Anne K. Soutar MRC Clinical Sciences Centre Imperial College Faculty of Medicine Hammersmith Hospital DuCane Road London, Null W12 ONN UNITED KINGDOM Phone: +44-208-383-2324 Fax: +44 208-383-2077 E-mail: anne.soutar@csc...
New drug enfuvirtide shows promise against drug resistant HIV infection
... who had had at least six months of treatment with retroviral
drugs or resistance to those drugs or both and also carried at least 5,000 copies of viral RNA per millimeter of plasma participated in the Western trial. Two-thirds of the nearly 500 patients received enfuvirtide plus a regimen of three to five anti...
Scientists: Cloak of human proteins gets HIV into cells
...Basic Biomedical Sciences. "But so many aspects of retroviral
biology have not been reconciled, including HIV, t...-- and more recent discoveries about exosomes. The retroviral
particles and exosomes contain the same human proteins, are built by the same machinery and have the...
Budding viral hijackers may co-opt cell machinery for the getaway
...he Chinese Academy of Sciences has shown that some retroviral
Gag proteins bind tightly to a host-cell protein called endophilin-2. Endophilin-2 is normally involved in the inward budding of the cell membrane, a process known as endocytosis. Supporting the idea that this interaction is functionally relevant, th...
Other highlights in the December 17 issue of JNCI
...ppearing in the December 17 JNCI: Interaction of retroviral
Tax oncoproteins with tristetraprolin and regulation of tumor necrosis factor-alpha expression, Luc Willems, Universitaire des Sciences Agronomiques de Gembloux, Gembloux, Belgium, et al. Effect of epidermal growth factor receptor inhibitor on develo...
RNA lariat may tie up loose ends to decades-old mystery of retrovirus life cycle
...tdoctoral researcherspursuing host-cell factors in retroviral
infections. In addition to filling a gap in biolo...here and helps explain some other anomalousdata on retroviral
DNA." While circumstantial evidence suggests the RNA lariat is the missing link, Menees is careful...
Transgenic animals produced using cultured sperm
... such as microinjection of genes into eggs and the retroviral
transduction of genes into embryos, often produce many animals that are mosaic, which means they do not contain the foreign gene in all their cells. In the case of zebrafish, less than 20 percent of fish produced by injecting DNA into fertilized eggs...
Primate viruses transmitted to people through bushmeat
...hunting and butchering of primates plays a role in retroviral
emergence. It is in all of our interests to put in...is suggests that, contrary to conventional wisdom, retroviral
zoonosis is widespread and arising from various locations where people are naturally exposed to mand...
New RNA libraries can selectively inactivate human genes
... The library of genes was integrated into a retroviral
vector that was capable of shuttling the genes int...e activity of a particular gene. But the retroviral
vectors used for shuttling the short hairpin RNAs into cells only went so far. They were not efficie...
Genetic trick adapted from viruses makes gene therapy vectors more versatile
...ts immune function. The St. Jude researchers used retroviral
vectors as the delivery system into which they ins...f DNA. The St. Jude team used these multicistronic retroviral
vectors (vectors carrying several different genes) to deliver the 2A peptide-linked CD3 gene cassett...
Friendly bacteria block HIV infection
...hrough mothers' milk," says Tao. "Unlike standard retroviral
drugs, which are too toxic for newborns, lactobacilli are 'friendly' bacteria already inhabiting the human digestive tract and milk products, and so should pose no danger to infants."...
Japanese researchers develop novel method of introducing transgenes into animals
...nic female mouse. Because stem cells treated with retroviral
transgenes can continuously generate large numbers of transgenic sperm, many transgenic offspring can be produced from a single "founder" male. In a paper accepted for publication in the journal Biology of Reproduction, the Japanese team notes that o...
'Search and destroy' protein turns tables on HIV
...3G) can directly mutate HIV. Such proteins--called retroviral
restrictors--may contribute to HIV resistance in s...ity factor), which triggers the destruction of the retroviral
restrictors, thereby preventing mutations from occurring. What scientists don't know is whether some...
Researchers in UGA Vet School discover a mechanism that blocks replication of a retrovirus
...es evolved and will help explain late steps in the retroviral
life cycle. The findings were published today in the Proceedings of the National Academy of Sciences. The team was led by Massimo Palmarini, a virologist at UGA's College of Veterinary Medicine. Co-authors are Manuela Mura, Pablo Murcia and Marco C...
Retroviral gene therapy? ASLV, HIV, and MLV show distinct target site preferences
...e therapy potential, Rick Mitchell et al. compared retroviral
vectors derived from three viruses and report 3,12...grate into the human genome by selecting different retroviral
integration systems. Only time will tell whether more control translates into safer gene therapy pro...