Watching DNA repair in real time
... of DNA repair, but very little is known about how assembly is controlled," said senior author Stephen Kowalczykowski, professor in the sections of Microbiology and of Molecular and Cellular Biology and director of the Center for Genetics and Development at UC Davis. Genes that control the human gene, Rad51, ...Structure determined for key molecular complex involved in long-term gene storage
...ular complex studied by the scientists governs the assembly of an especially condensed form of chromatin, the ...particular molecular partner, HIRA, when directing assembly of the more condensed form of chromatin. But it could also associate with a different partner, calle...Engineer ramps up protein production, develops versatile viral spheres
... proteins orstick to other capsids, allowing their assembly en masse. The tiny spheres could therefore transport targeted vaccines or medicines around the bloodstream or could be linked together in different configurations to make novel materials. Materials from assembled capsids could be light, strong and bi......covered a protein that plays a crucial role in the assembly and structure of the nucleus. Their work appears in the September 5 issue of Current Biology. The envelope acts as a barrier between the outer cell compartment, called the cytoplasm, and DNA stored in the cell nucleus. It regulates which molecules a...New anthrax inhibitor could combat antibiotic-resistant strains
...xin attaches in the body. Andbecause the nanoscale assembly of molecules is designed to bind tomultiple sites on the host receptor, it is naturally more potent. The new approach led to a 50,000-fold increase in potency in cellculture, and the inhibitor protected rats from anthrax toxin in thestudy. The genera...Be still my beating heart: Ilk gene underlies heart failure
...ular response. In particular, integrins direct the assembly of actin-based adhesion structures to propagate cellular forces, and are thus essential for cell migration, growth, and survival. Using two different animal model systems, Drs. Muller and Rottbauer show that loss of ILK in heart cells results in c...When the going gets tough, slime molds start synthesizing
...I PKS genes following the recently completed final assembly and annotation of the entire Dictyostelium genome, which was carried out by a worldwide collaboration of many scientists, including the Kay group. Working independently, Saito and Kay had deleted the Dictyostelium gene for Steely2. Not only could ...New light microscope sharpens scientists' focus
...ig, who is now a group leader at Janelia Farm. The assembly and underlying conceptual work on the microscope were personally funded with $25,000 each from Hess and Betzig. When Betzig and Hess began to develop the concept, the two scientists were unemployed. Both were in the midst of contemplating their car...Gene screen for breast cancer better than pathologist's 'eye'
...ation, which turns the gene switch off halting the assembly line of critical proteins found in normal cells. The Hopkins test, called quantitative multiplex methylation-specific PCR or QM-MSP, determines the percentage of methylation present in each of five to ten cancer genes. The percentages are added to...Researchers unveil strategy for creating actively-programmed anti-cancer molecules
...s In the study, the researchers used the unique assembly strategy to create a novel compound to combat meta...icated that this approach allows for the effective assembly of chemically programmed antibodies in vivo or in vitro, widening the possibilities for their therap...ACS Weekly PressPac -- June 26, 2006
...ew two other unrelated chemistry techniques -- the assembly of DNA micro-arrays and the stability of some forms of self-assembled monolayers. "The common thread is that all three technologies involve the deposition and/or presence of thin layers of liquid exposed to ambient air, so there are probably other me...Researchers map infectious hepatitis B virus
...his study little was known about the structure and assembly of native capsids present in infected cells in vivo, and even less was known about the structure of mature virions. "We used cryomicroscopy and image analysis to examine the native structure of HBV [hepatitis B virus ] capsids from transgenic mice a...The downside to diversification
... and cancer formation. V(D)J recombination is the assembly of the immune system's mature antigen-specific receptor genes through the rearrangement of separate V, D and J components in developing B and T cells. The RAG1 and RAG2 recombinase enzymes catalyze the initial DNA binding and cleavage, and occasional...Fox Chase study sheds light on cancer susceptibility and disease involving bone-marrow failure
...rotein defects to a critical glitch in the protein assembly line of ribosomes. This highlights the importance of proteomic research analyzing proteins. "While defects in a number of genes are known to lead to cancer and disease, this opens up a new avenue of research," Ruggero said. "The DNA may be fine, bu...Diverse sea 'bugs' revealed on landmark Atlantic cruise to census zooplankton
...ticular group, sorted through samples in a kind of assembly line "that would have made Henry Ford proud," according to University of Connecticut post-doctoral investigator Rob Jennings, leader of the on-board "Team DNA." Scientific highlights from the cruise The plankton captured includes tail-kicking shr...Scientists design potent anthrax toxin inhibitor
...ed liposome," hampers a critical early step in the assembly process that anthrax toxin must undergo to become fully active. In test-tube experiments, the inhibitor, which is covered with multiple short proteins (peptides), was 10,000 times more potent than unattached peptides. "If the effectiveness of anthrax...Anthrax inhibitor counteracts toxin, may lead to new therapeutics
...he University of Toronto have designed a nanoscale assembly of molecules that successfully counteracts and inh...rnal Nature Biotechnology, works by preventing the assembly of toxic enzyme components, thereby blocking the formation of fully assembled anthrax toxin and neut...In chemical genetics, a new strategy could speed drug discovery
...l1, which, when added to cell extracts, blocks the assembly of actin fibers used for cell motility. The method... protein individually to the mix until actin fiber assembly was restored. But this method is time-consuming and only works if the protein players in a biologic...DOE JGI to host Microbial Genomics Workshop for educators
..., will explore the tools that enable the order and assembly of the DNA fragments into strands that serve as starting material for the search for genes and other important features in microbial genomes. The workshop will also touch on the newly launched Integrated Microbial Genomes for metagenomic data (IMG/M......n the current work, the MIT team used an intricate assembly process to create the anode. Specifically, they ma...ab. "By harnessing the electrostatic nature of the assembly process with the functional properties of the virus, we can create highly ordered composite thin fil...