Tag: "duplex" at biology news
DNA constraints control structure of attached macromolecules
...ule, RNA." In previous DNA nanotechnology efforts, duplex
DNA has been used as a static lattice to construct geometrical objects in three dimensions. Instead of manipulating DNA alone into such shapes, the researchers are using DNA to control the folding and resulting structure of RNA. Eventually, they envi...
Clearing jams in copy machinery
...ert the now-single-stranded templates into two new duplex
DNA molecules. The new research shows that two different DNA polymerases, the high fidelity Pol III replicase and the low fidelity Pol IV, coordinate their action to cross obstacles encountered in the replication process. They attach themselves at th...
Evidence of rapid evolution is found at the tips of chromosomes
...d that TRF2, a shelterin protein that binds to the duplex
part of the telomere, is crucial for telomere protection. Without TRF2, telomeres activate a DNA damage signal and are repaired by the same pathways that act on DNA breaks. TRF2 brings a second shelterin protein, POT1, to the telomeres. Because POT1 ...
Lehigh scientist receives NHGRI grant to streamline, expand use of genome sequencing
...or example, about a stretching transition in a DNA duplex
that no one could have foreseen." Vezenov says his group will try to answer a scientific question of the ultimate resolution that one can achieve when measuring forces in biomolecules: Can we detect a difference of a single base? "If we can," he sa...
Stability of mRNA/DNA and DNA/DNA duplexes modulates mRNA transcription
...Stoynov. The mechanism of how DNA/DNA and mRNA/DNA duplex
stability influences mRNA level remains unclear. The authors propose two models, but further work is needed to understand how thermodynamic stability modulates mRNA level....
Triplex assay used to assay duplex genomic DNA
Direct detection of base sequence in duplex
nucleic acid has long been an unfulfilled objectiv...pathogens in samples also containing human genomic duplex
DNA and to the assay of SNPs present in human genomic samples. The assays are carried out homogeneou...
Hepatitis C helicase unwinds DNA in a spring-loaded, 3-step process
...ing double-stranded host DNA during infection. The duplex
created for the experiment included both single- and double-stranded DNA; fluorescent labels were located in the double-stranded region.) By tracking the gradually increasing distance between the two marked nucleotides as the strands separated in a...