Finding could improve safety of stem cell transplants
... "There already are ways to grow stem cells, purify them in cell culture and get a pure population of stem cells that you can transplant," says Dr. Condie. "You want to make those cells differentiate into a particular cell type that is no longer able to form teratomas," Dr. Bieberich says of this puri...USC-led team of scientists recreates DNA-mending pathway in test-tube
...re evolutionarily 'above' yeast, they first had to purify all the proteins used to rejoin the double strands of DNA once they've suffered damage and are severed from one another. As it turned out, two of the seven proteins come from a class of polymerases that were discovered in 1999 by Myron Goodman, Ph.D....Bayer donates equipment to Children's Hospital, Oakland for infectious disease research
...o study interactions between antibodies and cells, purify molecules and measure binding interactions between molecules that are available in only very small amounts," Moe said. "We're very pleased to benefit from the generosity of Bayer BP. The gift is a significant enhancement to the CIVD and will benefit ...Newly identified enzyme group converts protein into cellular traffic signal
...mical tour de force" by collaborators in France to purify it. In Gaertig's group, doctoral student Krzysztof Rogowski then identified the enzyme complex's active subunit and postdoctoral associate Dorota Wloga found genes for these enzymes in many organisms including humans. The researchers also showed tha......embrane protein complexes can be very difficult to purify in an intact form," says Jap. "Moreover, it's extr...xplains. "We wanted to isolate the native form and purify the whole gamma-secretase complex." An unexpected factor: Zhou and her colleagues isolated the...Masters' students in pharmacology do exciting research testing equipment and purifying DNA
...esearch showed a faster, cheaper and easier way to purify DNA. "DNA must be free from other contaminants after isolating it from a source such as plant, bacteria or virus cells," he said. The current method is often laborious and time consuming. If the extraction procedure could be incorporated onto a c...DOE JGI sequences DNA from extinct cave bear
...ally need genomic DNA, and nobody has been able to purify and sequence large quantities of DNA from these old samples. "We applied the standard techniques that we normally use for modern genome projects to ancient DNA specimens, a brute force high throughput sequencing approach. With this strategy, we were...U-M scientists discover identifying markers for primitive blood-forming stem cells
...intensive." "Many of the current markers we use to purify HSCs are expressed as a gradient from high to low across the hematopoietic cell hierarchy," says Omer H. Yilmaz, co-first author and a student in the Medical School's M.D./Ph.D. program. "What's really exciting about this finding is that now we have ...Identifying blood stem cells is a SLAM dunk
...recisely, differentially expressed that we can now purify HSCs much more simply and, in some contexts, much ... complicated combinations of markers to rigorously purify the stem cells that is too complicated to use in staining tissue sections." Using antibody stains t...Rensselaer researchers develop approach that predicts protein separation behavior
...Chromatography techniques are used to identify and purify molecules, in this case, particular proteins. "We intend to test the model against more complicated protein structures as part of its further development," said Breneman. "The outcome of this work will yield fundamental information about the complex ...System drastically cuts down botulism detection time
...vironmental samples. Next, an antibody is used to purify and concentrate the pathogen or toxin to enable accurate and sensitive detection. Finally, a second antibody, called a reporter antibody, labeled with a fluorescent dye or a fluorescent quantum dot binds to a different region on the toxin or pathoge...Leading lampreys to slaughter: Pheromone for scourge of Great Lakes identified
...his colleagues about 15 years to find, isolate and purify the pheromone so that Hoye and his colleagues could identify and synthesize it. The key component is a steroid with potency so great that lampreys would smell a single gram dissolved in 10 billion liters of water, enough to fill 5,000 Olympic-sized s...New antifreeze protein found in fleas may allow longer storage of transplant organs
...avies. "We use their affinity for ice as a tool to purify the protein." The antifreeze mechanism of snow fleas has been reported in other parts of the world, including Antarctica, but until now no one has isolated the protein. As well as its potential for use in organ transplants, the researchers suggest it...Pall Corporation presents approach for speeding vaccine production to Shanghai conference
...the use of disposable and scalable technologies to purify large molecule vectors, such as for flu vaccines, can help manufacturers streamline the process and overcome many of the unique challenges associated with vaccine development. Sellick's presentation reviewed advanced approaches to purification as par...Jefferson biologist coaxing human embryonic stem cells to make dopamine with simpler, faster method
...ich cells will be dopamine neurons and use them to purify cells before they are put into the animal. "That won't be easy, but this work gets us one step closer to it."...Scientists map one of biology's critical light-sensing structures
...o grow in the lab and their proteins are easier to purify and manipulate than plant proteins. Once isolated, the phytochrome was crystallized and its molecular structure was mapped using a beam of X-rays to develop a three-dimensional picture of the protein. That three-dimensional portrait, which reveals t...Plant-derived vaccines safeguard against deadly plague
...he plants to harvesting the plants. From there, we purify the antigens in an additional one to two weeks to create the vaccine." The approach also eliminates the typical year-long lag time necessary to establish and characterize genetically modified, or transgenic plants. The beauty of system is its pote...Berkeley scientists get first detailed look at Dicer
...fic genes. Doudna and her co-authors were able to purify and crystallize a Dicer enzyme from Giardia intestinalis, a one-celled microscopic parasite that can infect the intestines of humans and animals. This Dicer enzyme in Giardia is identical to the core of a Dicer enzyme in higher eukaryotes, including...UCR researchers design chip that can improve citrus varieties
...e of a credit card. To use the chip, researchers purify total RNA (which reflects the genes expressed in the tissue) from plant tissue, make a copy of these molecules with a chemical tag added, and then "wash" the chip with the RNA sample. If a gene is being expressed in the tissue, its corresponding RNA ...Yale's Strobel named 'million-dollar professor' for science teaching innovation
...n the world's rain forests. The students will then purify and analyze the compounds they collected and test them for potentially beneficial activity. Strobel's program will be an introductory science course for scientifically minded students, providing an engaging, hands-on learning experience that challeng...