Growth factor-promoting angiogenesis expressed in tumor cells and normal neurons
...chers demonstrate that decreased SCF expression in vivo results in decreased angiogenesis and improved survival in mouse glioma models, whereas overexpression of SCF is associated with a worse prognosis and shorter survival in patients with glioblastomas. SCF expression is not directly linked to tumor cel...Cancerous vs. healthy cells: Researchers identify the road to success
...PTEN contribute to trastuzumab resistance, both in vivo (humans and mice) and in vitro (culture). Patients with PTEN-deficient breast cancer have poorer outcomes and response to trastuzumab therapy when compared to those with normal PTEN levels. The researchers then examined the role played by phosphoi...News tips from The Journal of Neuroscience
...ine-coated surface or mature oligodendrocytes. In vivo , axonal growth cones made extensive contact with NG2 cells in the corpus callosum. Neurons extended processes on astrocytes or NG2 cells that were twice as long as those on fibroblasts or a polylysine surface. NG2 cells overexpressing NG2 did not i...Major obesity gene is 'lost in the shuffle'
...o identify the specific metabolic roles of ASIP in vivo by comparing a species that has the gene versus a species that lacks the gene," he says....Homeoprotein DNA-binding specificity
... SUMO E3 ubiquitin ligase protein underlies the in vivo DNA-binding specificity of the Msx1 homeoprotein. ...f how homeoproteins can find their target genes in vivo and, in addition, help pus to understand how the "address" of a gene in the nucleus contributes to i...New studies show topical glucosamine targets pigment overproduction
... overproduction in UV damaged skin cells. The in vivo and in vitro studies highlight improvements in hyperpigmentation, skin tone and barrier function. Data from the SIAscope, a non-invasive skin imaging system developed by Cambridge UK-based company Astron Clinica, enabled scientists to view the effec...News tips from the Journal of Neuroscience
...der MLD mice were less receptive to the cells. In vivo , the grafted cells differentiated into OLs and became myelinating. Most importantly, transplantation reduced sulfatide deposits by 2050% and normalized several measures of nerve function and behavior....JCI table of contents, March 16, 2006
...al ABCA1 directly contributes to HDL biogenesis in vivo AUTHOR CONTACT: Michael R. Hayden Centre for Molecular Medicine and Therapeutics, Vancouver, Canada Phone: (604) 875-3535; Fax: (604) 875-3819; E-mail: mrh@cmmt.ubc.ca View the PDF of this article at: https://www.the-jci.org/article.php?id=2...Biologists develop genome-wide map of miRNA-mRNA interactions
...re confirmed through the development of a novel in vivo method that asked whether the 3' UTR part of mRNAs...ional predictions, the NYU team developed a new in vivo analysis system comparing the expression of a reporter, green fluorescent protein (GFP) carrying tar...News tips from the Journal of Neuroscience
...t. This week, Olgun et al. considered its role in vivo during Xenopus laevis development. In embryos, the authors expressed a glucocorticoidinducible dominant-negative construct (dnXREST) that bound to the DNA of target genes but not corepressor proteins. In a second approach, REST translation was blo...Data published in PNAS show antibodies can be made 10 times more toxic to cancer cells
...n two orders of magnitude. Studies conducted in in vivo models demonstrated that these antibodies were greater than ten times more toxic to target cells. The enhanced antibodies also were able to kill tumor cells that are typically "invisible" to other antibodies because they express low levels of target...Carnegie Mellon study identifies impact of neural connections in learning process
... of synaptic plasticity to how neurons function in vivo is critical to advancing our knowledge of the mech...ins Barth. "Evidence from in vitro studies and in vivo studies using viral over-expression systems supports the notion that production of certain AMPA rece...Experimental drug reverses key cognitive deficits, pathology in Alzheimer's
...a, Irvine and his colleagues reported the first in vivo studies of the drug's effects. AF267B was developed by coauthor Abraham Fisher to activate particular receptors for the neurotransmitter acetylcholine. These specific receptors, called M1 receptors, are abundant in areas of the brain--the cortex and ...JCI table of contents, February 23, 2006
...ing antibodies inhibit IgE-mediated anaphylaxis in vivo through both antigen interception and Fc(gamma)RIIb cross-linking AUTHOR CONTACT: Fred D. Finkelman University of Cincinnati, Cincinnati, Ohio, USA Phone: (513) 558-4701; Fax: (513) 558-3799; E-mail: ffinkelman@pol.net View the PDF of this article ...New study shows antibody-interleukin complexes stimulate immune responses
...) expands the proliferation of specific T cells in vivo by increasing the biological activity of naturally occurring IL-2 through the formation of immune complexes. When combined with recombinant IL-2, some IL-2/IL-2 mAb complexes cause more than a 100-fold proliferation in CD8+ T cells, which can target ...Combination of PET, MR imaging shows white matter degeneration in Huntington's disease patients
... he said. "For the first time, we are providing in vivo evidence that glial cells--the supportive cells in the central nervous system--are involved in early disease," he noted. More research needs to be done, and scientists need "to go through the basic science to find the real biological cause of such an...News tips from the Journal of Neuroscience
...xpression of Dab1 in migratingcortical neurons in vivo using RNAi. Normally, cells migratedthrough the cell-dense corticalplate (CP) and entered the marginal zone(MZ), where the leading process typicallybecame branched. At embryonic day 20 inlateral neocortex and cingulate cortex,Dab1-deficient neuronal...Post-pregnancy events promote breast tumor metastasis
...gh virgin matrix. Schedins group next performed in vivo experiments to further confirm that post-lactation involution matrix enhances tumor cell migration (i.e. metastasis). Breast tumor cells were mixed with either involution or virgin matrix, and the mixtures were injected into the mammary fat pads of m...New route to therapy for Rett syndrome?
...n--noted the researchers. "Our findings provide in vivo evidence of a functional interaction between Mecp2 and Bdnf," concluded the researchers. "Furthermore, our results indicate that manipulating the BDNF level or the BDNF signaling pathways may present therapeutic opportunities for [Rett syndrome] pati...Brain derived neurotrophic factor alleviates disease symptoms in a mouse model of Rett Syndrome
...tating symptoms seen in RTT. To investigate the in vivo role (in a living organism) of BDNF in RTT Qiang Chang, a post-doctoral fellow in the Jaenisch lab, manipulated BDNF levels in the brains of genetically engineered "Rett mice". He discovered that deleting bdnf from the Mecp2 mutant mice resulted in ...