Study finds HIV protease inhibitor drugs may adversely affect the scaffolding of the cell nucleus
... UCLA researchers added HIV protease inhibitors to cultures of mouse and human fibroblast cells. They found that the inhibition of ZMPSTE24 by the HIV protease inhibitor drugs led to an accumulation of prelamin A, which is a precursor to mature lamin A a key molecule in the structural scaffolding for the cel...Taking animals out of laboratory research
...er with validated alternative methods such as cell cultures and computer modelling. The charity is seeking reliable, scientifically proven ways forward which take account of the welfare of both humans and animals, without jeopardising the ground-breaking work on the major healthcare challenges of the 21st c...Community-associated staph infections involving antibiotic-resistant bacteria increase
...k County, Chicago, examined tissue, fluid and bone cultures at the 464-bed public hospital and its associated clinics between 2000 and 2005. They restricted their study to patients with infections of community onset, not hospital onset, to understand infection rates in the community; patients studied were tho...Syphilis screening, lithium fights neurodegeneration, monoclonal antibodies against avian flu
...Finally, the authors showed that infection of cell cultures of astrocyes infected with HHV-6 showed a marked decrease in the expression of one protein, EAAT, which is known to be involved in transmitting glutamate across cell membranes; glutamate is a substance that acts to carry signals within the brain. O...New therapeutic targets for neurodegenerative diseases
...Neurobiology Laboratory are investigating cells in cultures - neurones, oligodendrocytes or other cells of the nervous system -, and are trying to reproduce in vitro circumstances that are thought to be relevant in these ailments. That is to say, they are creating the conditions that cause the death of these ...Culture sculpts neural response to visual stimuli, new research indicates
...g and retrieval. As expected, older adults in both cultures exhibited diminished binding processes (the ability to connect a particular object to its background) in the hippocampus, as compared with younger study subjects. The older subjects also exhibited diminished object processing in the lateral occipital...University of Alberta hosts international conference on women in the knowledge economy and society
...erence addressing the features of local and global cultures that encourage and impede women's active, creative and critical participation in the knowledge economy. Rooms of Their Own: Women in the Knowledge Economy and Society will run from Wednesday, May 2 to Friday, May 4 at the Telus Centre on the Universi...Johns Hopkins begins aggressive screening for 'superbugs' in children
... pain. Weekly swab testing and bacterial growth cultures were done on nearly 330 patients in the hospital's...four months. Results were compared to findings of cultures obtained from patients showing possible signs or symptoms of infection. All patients were under age...Blood marker helps predict prognosis among those with abdominal infection
...abdomens of all participants, as were other tissue cultures when sepsis was suspected. The patients were monitored from within 96 hours of their first symptoms to a maximum of 21 days for levels of procalcitonin and another marker of inflammation, C-reactive protein, and for signs of lung or kidney failure. ...Clinical guidelines help identify which children at risk of bacterial meningitis
...erial meningitis requires negative CSF (and blood) cultures after 2 to 3 days of incubation, most children with CSF pleocytosis are admitted to the hospital to receive broad-spectrum antibiotics while awaiting culture test results. A highly accurate decision support tool that could identify which children wi...Structure essential for brain remodeling identified
...ulated the neurons with chemicals and examined the cultures using a technique called live-cell imaging, in which a camera attached to a powerful microscope recorded the dendritic spines as they grew. This technique, Ehlers said, enabled the team to glimpse inside the internal world of the neuron to see how th...U-M researchers use nanoparticles to target brain cancer
...r. Researchers tested the nanoparticles in cell cultures and animal models. The studies showed the nanoparticles traveled to the tumor, resulting in less Photofrin exposure throughout the body and enhanced exposure within the tumor. This allowed a larger window for activating the drug with light. It also w...Faster, more accurate tuberculosis test developed
...e samples using MODS, the researchers observed the cultures under an inverted light microscope for 40 days. Th...thambutol and streptomycin--TB antibiotics--to the cultures in order to detect drug-resistant TB. Compared to standard culture reference methods, drug-resistant...Researchers identify new weapon to fight deadly bacterial sepsis
... to survive. Other experiments conducted in cell cultures traced the specific biochemical pathways by which LPS triggers an inflammatory cycle that damages the cells of the heart. By blocking aldose reductase activity with pharmacological inhibitors or using small interfering RNAs (pieces of genetic materia...Transmission of antibiotic-resistant bacteria linked to previous intensive care unit room occupants
...2005. As part of the hospitals' normal protocols, cultures were obtained from all ICU patients when they arrived and every week they stayed to determine the presence of MRSA and VRE. Upon entering the ICU, 809 patients carried MRSA and 658 carried VRE, leaving 7,629 to screen for the acquisition of MRSA a...Therapeutic role found for carbon monoxide
...s Otterbein. But, he adds, when they exposed these cultures to carbon monoxide, nothing happened. The cells behaved normally. "We eventually asked ourselves, 'What if we're not seeing results in these two separate dishes because in the body, the two aren't separated. What if these two cell types somehow act ...TB relapse due to low weight gain after initial treatment
... who had abnormal chest x-rays and positive sputum cultures after 2 months of anti-tuberculosis treatment. "Less than 5 percent weight gain could be a marker of increased tuberculosis disease activity and/or poor response to therapy," said Dr. Khan. TB is a contagious, potentially fatal infection usually...New sleep gene discovery wakes up scientists
...t Forger's simulation. The researchers took cell cultures and observed that for those with the mutated gene, the protein only took a couple hours to degrade. For the normal gene, it took 8-10 hours. Next, Virshup said, his team will begin testing ways to regulate the circadian rhythm in mice, a necessary...Experiments with fruit quality improvement lead to new approach for halting spread of cancer cells
...e effects. In laboratory experiments using cell cultures that originated from human colon cancer, breast cancer and melanoma, increasing the level of actibind was found to reduce the ability of these cells to form tumorogenic colonies. Further experimentation, with a variety of animal models, showed that t...... in the lab. Therefore, the results from bacterial cultures are not considered central to the clinical care of the patient. At the start of the study, all participants were deemed similar with respect to the pneumonia severity index and clinical, laboratory-related and microbiological characteristics. B...